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Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

文献类型： 外文期刊

作者： Zhao, Zihan 1 ; Liu, Yue 1 ; Zhang, Yushi 1 ; Geng, Zeyu 1 ; Su, Rina 1 ; Zhou, Lipeng 1 ; Han, Chao 1 ; Wang, Zhanjun 2 ; Ma, Shuangcheng 3 ; Li, Weidong 1 ;

作者机构： 1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 102488, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Forestry & Grassland Ecol, Yinchuan 750002, Peoples R China

3.Natl Inst Food & Drug Control, Inst Control Chinese Tradit Med & Ethn Med, Beijing 100050, Peoples R China

关键词： Pruni semen; Acetyl flavonoids; Amygdalin; Multivariate analyses; Authentic sources

期刊名称：JOURNAL OF PHARMACEUTICAL ANALYSIS （ 影响因子：8.8； 五年影响因子：7.2 ）

ISSN： 2095-1779

年卷期： 2022 年 12 卷 5 期

页码：

收录情况： SCI

摘要： Pruni Semen, the seed of several unique Prunus plants, is a traditional purgative herbal material. To determine the authentic sources of Pruni Semen, 46 samples from four species were collected and analyzed. Ten compounds including multiflorin A (Mul A), a notable purative compound, were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy. Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry, and acetyl flavonoid glycosides were recognized as characteristic constituents. The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel. Based on this, methods for identifying Pruni Semen from different sources were established using chemical fingerprinting, quantitative analysis of the eight principal compounds, hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The results showed that the samples were divided into two categories: one is the small seeds from Prunus humilis (Ph) and Prunus japonica (Pj), and the other is the big seeds from Prunus pedunculata (Pp) and Prunus triloba (Pt). The average content of Mul A was 3.02, 6.93, 0.40, and 0.29 mg/g, while the average content of amygdalin was 18.5, 17.7, 31.5, and 30.9 mg/g in Ph, Pj, Pp, and Pt, respectively. All the above information suggests that small seeds might be superior sources of Pruni Semen. This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of Xi'an Jiaotong University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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