文献类型： 外文期刊

作者： Li, Yanan ¹ ; Wang, Chengyu ¹ ; Wang, Tianye ¹ ; Liu, Yutao ³ ; Jia, Shuxia ⁴ ; Gao, Yunhang ⁵ ; Liu, Shuxia ¹ ;

作者机构： 1.Jilin Agr Univ, Coll Resources & Environm, Changchun 130000, Peoples R China

2.Key Lab Soil Resource Sustainable Utilizat Jilin, Changchun 130000, Peoples R China

3.Heilongjiang Acad Agr Sci, Qiqihar Branch Inst, Qiqihar 161000, Peoples R China

4.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Changchun 130102, Jilin, Peoples R China

5.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130000, Jilin, Peoples R China

关键词： metagenomic analysis; rhizosphere; soil microbiome; microbiome function; fertilizer

期刊名称：LAND （ 影响因子：3.9； 五年影响因子：4.0 ）

ISSN：

年卷期： 2020 年 9 卷 9 期

页码：

收录情况： SCI

摘要： Fertilization influences the soil microbiome. However, little is known about the effects of long-term fertilization on soil microbial metabolic pathways. In this study, we investigated the soil microbiome composition and function and microbial participation in the N cycle according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) functional annotation of different genes in a metagenomic analysis after long-term fertilization. Fertilizer application significantly changed the soil C/N ratio. Chemical fertilizer (NPK) treatment decreased soil pH, and chemical fertilizer combined with straw (NPK+S0.5) treatment increased ammonium nitrogen (NH4+-N) but decreased nitrate nitrogen (NO3--N). NPK, NPK+S0.5 and S0.5 applications did not change the soil microbiome composition or dominant phylum but changed the relative abundances of microbiome components. Moreover, fertilizer significantly influenced metabolic processes, cellular processes and single-organism processes. Compared with a no-fertilizer treatment (CK), the NPK treatment resulted in more differentially expressed gene (DEG) pathways than the NPK+S0.5 and S0.5 treatments, and these pathways significantly correlated with soil nitrate nitrogen (NO3--N), available phosphorus (AP) and the moisture content of soil (MC). KEGG analysis found that fertilizer application mainly affected the ribosome, photosynthesis and oxidative phosphorylation pathways. S0.5 and NPK+S0.5 increased microbial nitrogen fixation, and NPK and NPK+S0.5 decreased amoA and amoB and accelerated denitrification. Thus, organic fertilizer increased N fixation and nitrification, and inorganic N fertilizer accelerated denitrification. We found that the function of the soil microbiome under different fertilizer applications could be important for the rational application of fertilizer and for environmental and sustainable development.