文献类型： 外文期刊

作者： Li, Peihuan ¹ ; Chen, Jiale ¹ ; Ying, Shumin ¹ ; Chen, Nike ¹ ; Fang, Shu ¹ ; Ye, Menglei ¹ ; Zhang, Chunhua ² ; Li, Chonghua ³ ; Ge, Ying ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Lab Ctr Life Sci, Demonstrat Lab Element & Life Sci Res, Nanjing 210095, Peoples R China

3.Guangdong Acad Agr Sci, Inst Agr Resources & Environm, Guangzhou 510640, Peoples R China

关键词： Sinorhizobium sp.; Pb; Zn; Tolerance; EPS

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：8.9； 五年影响因子：9.5 ）

ISSN： 0269-7491

年卷期： 2024 年 343 卷

页码：

收录情况： SCI

摘要： Lead (Pb) and zinc (Zn) have been discharged into environment and may negatively impact ecological security. Rhizobia has gained attention due to their involvement in the restoration of metal polluted soils. However, little is known about the responses of rhizobia under Pb and Zn stress, especially the roles played by extracellular polymeric substances (EPS) in the resistance of these two metals. Here, Sinorhizobium sp. C10 was isolated from soil around a mining area and was exposed to a series of Pb/Zn treatments. The cell morphology and surface mineral crystals, EPS content and fluorescent substances were determined. In addition, the extracellular polysaccharides and proteins were characterized by attenuated total reflection infrared spectroscopy (ATR-IR) and Xray photoelectron spectroscopy (XPS). The results showed that Zn stress induced the synthesis of EPS by C10 cells. Functional groups of polysaccharides (C--O) and proteins (C-O/C-N) were involved in complexation with Zn. In contrast, C10 resisted Pb stress by forming lead phosphate (Pb3(PO4)2) on the cell surface. Galactose (Gal) and tyrosine played key roles in resistance to the Zn toxicity, whereas glucosamine (N-Glc) was converted to glucose in large amounts during extracellular Pb precipitation. Together, this study demonstrated that C10 possessed different strategies to detoxify the two metals, and could provide basis for bioremediation of Pb and Zn polluted sites.