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Creation and gene expression analysis of a giant embryo rice mutant with high GABA content

文献类型： 外文期刊

作者： Chen, Pingli 1 ; Liu, Qing 1 ; Sun, Bingrui 1 ; Lv, Shuwei 1 ; Jiang, Liqun 1 ; Zhang, Jing 1 ; Mao, Xingxue 1 ; Yu, Hang 1 ; Chen, Yangyang 1 ; Chen, Wenfeng 1 ; Fan, Zhilan 1 ; Pan, Dajian 1 ; Li, Chen 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Key Lab New Technol Rice Breeding,Minist, Guangdong Rice Engn Lab,Key Lab Genet & Breeding H, Guangzhou 510640, Peoples R China

关键词： Gamma-amino butyric acid (GABA); Giant embryo rice; CRISPR/Cas9; RNA-seq; Grain development

期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 1380-3743

年卷期： 2023 年 43 卷 1 期

页码：

收录情况： SCI

摘要： Gamma-amino butyric acid (GABA) is a natural non-protein amino acid involved in stress, signal transmission, carbon and nitrogen balance, and other physiological processes in plants. In the human body, GABA has the effects of lowering blood pressure, anti-aging, and activating the liver and kidneys. However, there are few studies on the molecular regulation mechanism of genes in the metabolic pathways of GABA during grain development of giant embryo rice with high GABA content. In this study, three glant embryo (ge) mutants of different embryo sizes were obtained by CRISPR/Cas9 knockout, and it was found that GABA, protein, crude fat, and various mineral contents of the ge mutants were significantly increased. RNA-seq and qRT-PCR analysis showed that in the GABA shunt and polyamine degradation pathways, the expression levels of most of the genes encoding enzymes promoting GABA accumulation were significantly upregulated in the ge-1 mutant, whereas, the expression levels of most of the genes encoding enzymes involved GABA degradation were significantly downregulated in the ge-1 mutant. This is most likely responsible for the significant increase in GABA content of the ge mutant. These results help reveal the molecular regulatory network of GABA metabolism in giant embryo rice and provide a theoretical basis for the study of its development mechanisms, which is conducive to the rapid cultivation of GABA-rich rice varieties, promoting human nutrition, and ensuring health.

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