您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

In Silico Characterization of bla(NDM)-Harboring Conjugative Plasmids in Acinetobacter Species

文献类型： 外文期刊

作者： Tang, Biao 1 ; Wang, Chenyu 3 ; Sun, Dongchang 3 ; Lin, Hui 1 ; Ma, Jiangang 1 ; Guo, Hengzhao 4 ; Yang, Hua 1 ; Li, Xiaobin 5 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou, Zhejiang, Peoples R China

3.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou, Peoples R China

4.Jinan Univ, Dept Radiat Oncol, Zhuhai Peoples Hosp, Zhuhai Hosp, Zhuhai, Guangdong, Peoples R China

5.Jinan Univ, Zhuhai Precis Med Ctr, Zhuhai Peoples Hosp, Zhuhai Hosp, Zhuhai, Guangdong, Peoples R China

关键词： Acinetobacter; New Delhi metallo-beta-lactamase; plasmid; conjugative

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2022 年 10 卷 6 期

页码：

收录情况： SCI

摘要： New Delhi metallo- beta- lactamase (NDM)-producing clinical strains in Acinetobacter spp. have been recently reported in many countries and have received considerable attention. The vast majority of bla(NDM) cases occur on conjugative plasmids, which play a vital role in disseminating bla(NDM). To characterize the conjugative plasmids bearing blaNDM genes in Acinetobacter spp., we analyzed the variants of bla(NDM), conjugative transfer regions, genetic contexts of bla(NDM), and the phylogenetic pattern of the 62 predicted blaNDM-positive plasmids, which were selected from 1,191 plasmids of Acinetobacter species from GenBank. We identified 30 conjugative plasmids from the 62 bla(NDM)-harboring plasmids in Acinetobacter species, with the oriT sites similar to plasmid pNDM-YR7 in our study, genes coding for relaxases of the MOBQ family, genes encoding type IV coupling proteins (T4CPs) of the TrwB/TraD subfamily, and VirB-like type IV secretion system (T4SS) gene clusters. The genome sizes of all 30 pNDM-YR7-like plasmids ranged from 39.36 kb to 49.65 kb, with a median size of 44.56 kb. The most common species of Acinetobacter containing the blaNDM-positive conjugative plasmids was A. baumannii, followed by Acinetobacter lwoffii and Acinetobacter indicus. Notably, pNDM-YR7 is the first report on a bla(NDM)-positive conjugative plasmid in Acinetobacter junii. Moreover, all 30 bla(NDM)-positive conjugative plasmids in Acinetobacter species were found to contain genetic contexts with the structure ISAba14-aph(3')-VI-ISAba125-bla(NDM)-ble. Our findings provide important insights into the phylogeny and evolution of bla(NDM)-positive plasmids of Acinetobacter species and further address their role in acquiring and spreading bla(NDM) genes in Acinetobacter species.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台