Transcriptomic Insights into Dual Temperature-Salinity Stress Response in "Shuike No. 1", a Pioneering Rainbow Trout Strain Bred in China
文献类型： 外文期刊
作者： Liu, Xiaojun 1 ; Wang, Gaochao 1 ; Huang, Tianqing 1 ; Liu, Enhui 1 ; Gu, Wei 1 ; Fan, Peng 1 ; Ge, Kaibo 1 ; Li, Datian 1 ; Sun, Yunchao 1 ; Xu, Gefeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Biotechnol & Breeding, Harbin 150070, Peoples R China
3.Engn Technol Res Ctr Cold Water Fish Breeding Heil, Harbin 150070, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： rainbow trout; salinity stress; temperature stress; RNA-seq; WGCNA
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 1 期
页码：
收录情况： SCI
摘要： Global warming poses a significant threat to aquaculture, particularly for cold-water species like rainbow trout (Oncorhynchus mykiss). Understanding the molecular mechanisms underlying stress responses is crucial for developing resilient strains. This study investigates the dual stress of salinity and temperature response of "Shuike No. 1" (SK), a pioneering commercially bred rainbow trout strain in China, using RNA-sequencing of gill, intestine, and liver tissues from fish exposed to four treatment combinations: freshwater at 16 degrees C, freshwater at 25 degrees C, saltwater (30 parts per thousand) at 16 degrees C, and saltwater at 25 degrees C. Differential gene expression analysis identified a substantial number of DEGs, with the liver showing the most pronounced response and a clear synergistic effect observed under combined high-temperature and salinity stress. Weighted gene co-expression network analysis (WGCNA) revealed stress-responsive gene modules and identified hub genes, primarily associated with gene expression, endoplasmic reticulum (ER) function, disease immunity, energy metabolism, and substance transport. Key hub genes included klf9, fkbp5a, fkbp5b, ef2, cirbp, atp1b1, atp1b2, foxi3b, smoc1, and arf1. Functional enrichment analysis confirmed the prominent role of ER stress, particularly the pathway "protein processing in the endoplasmic reticulum." Our results reveal complex, tissue-specific responses to dual stress, with high temperature exerting a stronger influence than salinity. These findings provide valuable insights into the molecular mechanisms underpinning dual stress responses in SK, informing future breeding programs for enhanced resilience in the face of climate change.
- 相关文献
作者其他论文 更多>>
-
Whole-genome resequencing reveals selection signatures in China's first rainbow trout (Oncorhynchus mykiss) strain "Shuike No. 1"
作者：Ma, Haibing;Wang, Gaochao;Huang, Tianqing;Liu, Enhui;Gu, Wei;Xu, Gefeng;Ma, Haibing;Xu, Gefeng
关键词：Rainbow trout; Population structure; Selective sweep; Linkage disequilibrium
-
Comprehensive Assessment of Rapeseed Meal as a Fish Meal Substitute in Hybrid Sturgeon (Acipenser schrenckii ♀ x Acipenser baerii ♂) Diets: Impacts on Growth Performance, Body Composition, Immunological Responses, Intestinal Histology, and Inflammatory Response
作者：Zhang, Wenpeng;Liu, Siyuan;Wang, Shidi;Wang, Huamin;Ge, Kaibo;Lu, Shaoxia;Han, Shicheng;Wang, Chang'an;Liu, Hongbai;Zhang, Wenpeng;Ge, Kaibo;Yang, Yuhong;Wang, Chang'an;Liu, Siyuan;Wang, Shidi;Wang, Huamin;Jiang, Haibo
关键词：growth performance; hybrid sturgeon; immunological response; intestinal histology; rapeseed meal
-
Functional Analysis of the Cyclin E Gene in the Reproductive Development of Rainbow Trout (Oncorhynchus mykiss)
作者：Liu, Enhui;Song, Haixia;Gu, Wei;Wang, Gaochao;Fan, Peng;Ge, Kaibo;Sun, Yunchao;Li, Datian;Xu, Gefeng;Huang, Tianqing;Liu, Enhui;Song, Haixia;Gu, Wei;Wang, Gaochao;Fan, Peng;Ge, Kaibo;Sun, Yunchao;Li, Datian;Xu, Gefeng;Huang, Tianqing;Song, Haixia
关键词：rainbow trout;
Cyclin E ; meiosis; reproduction -
Effects of alanine supplementation on the growth, biochemical composition and appetite of ivory shell (Babylonia areolata)
作者：Hong, Xin;Sun, Yunchao;Liu, Jingcan;Yu, Feng;Yang, Yi;Liu, Chunsheng;Sun, Yunchao;Yu, Feng;Yang, Yi;Liu, Chunsheng
关键词：Babylonia areolata; Alanine supplementation; Growth performance; Amino acid composition; Appetite
-
Long Non-Coding RNA 74687 Regulates Meiotic Progression and Gonadal Development in Rainbow Trout (Oncorhynchus mykiss) via the miR-15a-5p-ccne1 Regulatory Axis
作者：Huang, Tianqing;Cao, Baorui;Liu, Enhui;Gu, Wei;Sun, Yunchao;Ge, Kaibo;Wang, Gaochao;Li, Datian;Fan, Peng;Xing, Ruiyan;Xu, Gefeng;Cao, Baorui
关键词：lncRNA; ccne1; miR-15a-5p; gonadal development; meiosis
-
Protective effects of polysaccharide from Atractylodes macrocephala Koidze (PAMK) as a feed additive on growth performance, immunity, antioxidant capacity, and disease resistance against Aeromonas salmonicida in rainbow trout (Oncorhynchus mykiss)
作者：Lu, Shaoxia;Wang, Chang'an;Wang, Di;Shi, Honghe;Wang, Shidi;Xu, Gefeng;Liu, Hongbai;Han, Shicheng;Lu, Shaoxia;Wang, Di;Shi, Honghe;Wang, Shidi;Liu, Hongbai;Han, Shicheng;Bian, Xunwen
关键词：Polysaccharide from Atractylodes macrocephala koidze (PAMK); Antioxidant capacity; Immune response; Oncorhynchus mykiss
-
Influence of increasing acclimation temperature on growth, digestion, antioxidant capacity, liver transcriptome and intestinal microflora of Ussruri whitefish Coregonus ussuriensis Berg
作者：Ge, Kaibo;Fan, Ze;Huang, Tianqing;Gu, Wei;Wang, Gaochao;Liu, Enhui;Pan, Runlei;Li, Datian;Sun, Yunchao;Yao, Zuochun;Wang, Liwei;Zhao, Cheng;Xu, Gefeng;Ge, Kaibo;Huang, Tianqing;Gu, Wei;Wang, Gaochao;Liu, Enhui;Pan, Runlei;Li, Datian;Sun, Yunchao;Yao, Zuochun;Xu, Gefeng;Fan, Ze
关键词：Ussruri whitefish Coregonus ussuriensis Berg; Temperature; Growth performance; Liver transcriptional responses; Intestinal microflora
