Identification and characterization of ClAPRR2, a key candidate gene controlling watermelon stripe color
文献类型： 外文期刊
作者： Liang, Shuang 1 ; Yang, Miaomiao 1 ; Zhang, Linlin 1 ; Fang, Xufeng 1 ; Zhang, Xian 3 ; Wei, Chunhua 3 ; Dai, Zuyun 4 ; Yang, Zhongzhou 4 ; Wang, Chaonan 5 ; Liu, Bin 6 ; Luan, Feishi 1 ; Liu, Shi 1 ;
作者机构： 1.Northeast Agr Univ, Coll Hort & Landscape Architecture, Key Lab Biol & Genet Improvement Hort Crops Northe, Minist Agr & Rural Affairs, Harbin 150030, Peoples R China
2.Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China
3.Northwest A&F Univ, Coll Hort, Yangling 712100, Peoples R China
4.Anhui Jianghuai Hort Technol Co Ltd, Hefei 230031, Peoples R China
5.Xinjiang Agr Univ, Coll Hort, Urumqi 830052, Peoples R China
6.Xinjiang Acad Agr Sci, Hami Melon Res Ctr, Urumqi, Peoples R China
关键词： Watermelon; Stripe color; Genetic mapping; Chlorophyll
期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）
ISSN： 0168-9452
年卷期： 2025 年 352 卷
页码：
收录情况： SCI
摘要： The stripe color of watermelon is a vital commercial trait and is the focus of attention of consumers and researchers. However, the genetic determinants of watermelon stripe color are incompletely understood. Based on the results of preliminary localization studies, we constructed a large-capacity F2 generation population (710 plants) using light-green striped ZXG1555 and green-striped Cream of Saskatchewan (COS) watermelon strains as parental lines for fine mapping. Genes controlling stripe color were located in an 85.284 kb region on chromosome 9, which contained five candidate genes. Combined with parental phenotypes, chlorophyll contents of rinds and stripes were assayed. Gene sequence alignment and transcriptional level analysis of parental lines predicted Cla97C09G175170 (encoding a two-component response regulator-like protein, APRR2) as the best candidate gene for stripe color trait. Two SNPs in the ClAPRR2 coding region caused amino acid substitutions, but were not located in the conserved domain, while a 12 bp insertion caused premature translation termination and a 35 amino acid deletion in the conserved domain and may have affected ClAPRR2 function in ZXG1555. Subcellular localization analysis showed that ClAPRR2 was expressed in the ZXG1555 cell membrane but was located in the nucleus and cell membrane of COS. Nucleotide polymorphisms and deletions were also detected in the promoter region between parental lines and caused cis-acting element variations. Luciferase activity suggested that promoter variations may not be the main factor in the regulation of ClAPRR2 expression.
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