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A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis

文献类型： 外文期刊

作者： Shi, Ke 1 ; Wang, Wei 2 ; Sun, Jingjing 2 ; Jiang, Chengcheng 2 ; Hao, Jianhua 2 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Drugs & Byproducts,Natl Lab Marine Sci, Pilot Natl Lab Marine Sci & Technol,State Key Lab, Qingdao 266071, Peoples R China

3.Jiangsu Collaborat Innovat Ctr Exploitat & Utiliza, Lianyungang 222005, Peoples R China

关键词： C-phycocyanin; Spirulina platensis; Affinity chromatography; Virtual screening

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.1； 五年影响因子：3.7 ）

ISSN： 0021-9673

年卷期： 2024 年 1720 卷

页码：

收录情况： SCI

摘要： The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent. With this customized and synthesized resin, a highefficiency one-step purification procedure for C-phycocyanin was developed and optimized, the purity was determined to be 4.53, and the yield was 69 %. This one-step purification protocol provides a new approach for purifying other phycobilin proteins.

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