Characterization of a Natural Accession of Elymus sibiricus with In Situ Hybridization and Agronomic Evaluation
文献类型： 外文期刊
作者： Liu, Yizhuo 1 ; Ding, Jiarui 1 ; Wu, Chunfei 1 ; Song, Weiwei 1 ; Zhao, Xinyu 1 ; Zhao, Haibin 2 ; Qu, Yunfeng 3 ; Jin, Hui 2 ; Zhang, Rui 2 ; Li, Mingyao 1 ; Yan, Xinyu 1 ; Zhu, Liangyu 1 ; Bao, Yaqi 1 ; Liu, Dianhao 1 ; Li, Xinling 1 ; Cui, Lei 4 ; Li, Hongjie 3 ; Zhang, Yanming 1 ;
作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, Lab Mol Cytogenet & Genet Breeding Heilongjiang Pr, Harbin 150025, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, Harbin 150086, Peoples R China
3.Xianghu Lab, Inst Biotechnol, Hangzhou 311200, Peoples R China
4.Shanxi Agr Univ, Coll Agr, Taiyuan 030031, Peoples R China
关键词：
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 1 期
页码：
收录情况： SCI
摘要： Elymus sibiricus, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the E. sibiricus accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic in situ hybridization and multicolor fluorescence in situ hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts.
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