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Natural variation of the Dt2 promoter controls plant height and node number in semi-determinant soybean

文献类型： 外文期刊

作者： Kou, Kun 1 ; Su, Tong 1 ; Wang, Yanping 3 ; Yang, Hui 4 ; Du, Hao 4 ; He, Milan 4 ; Li, Tai 4 ; Ma, Lixin 4 ; Liao, Chunmei; 1 ;

作者机构： 1.Chinese Acad Sci, Innovat Acad Seed Design, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin, Peoples R China

2.Univ Chinese Acad Sci, Beijing, Peoples R China

3.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang, Peoples R China

4.Guangzhou Univ, Innovat Ctr Mol Genet & Evolut, Sch Life Sci, Guangzhou, Peoples R China

关键词： Soybean; QTL; Genotyping-by-sequencing (GBS); Plant height; Node number; Dt2

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2021 年 41 卷 6 期

页码：

收录情况： SCI

摘要： Soybean (Glycine max (L.) Merrill) is an important legume crop worldwide. Plant height (PH) is a quantitative trait that is closely related to node number (NN) and internode length (IL) on the main stem, which together affect soybean yield. To identify candidate genes controlling these three traits in soybean, we examined a recombinant inbred line (RIL) population derived from a cross between two soybean varieties with semi-determinate stems (Dt1Dt1Dt2Dt2), JKK378 and HXW. A quantitative trait locus (QTL) named qPH18 was identified that simultaneously controls PH, NN, and IL; this region harbors the semi-determinant gene Dt2. Sequencing of the Dt2 promoter from JKK378 identified three polymorphisms relative to HXW, including two single nucleotide polymorphism (SNPs) and an 18-bp insertion/deletion polymorphism (Indel). Dt2 expression was lower in the qPH18(JKK378) group than in the qPH18(HXW) group, whereas the expression level of the downstream gene Dt1 showed the opposite tendency. A transient transfection assay confirmed that Dt2 promoter activity is lower in JKK378 compared to HXW. We propose that the polymorphisms in the dominant Dt2 promoter underlie the differences in Dt2 expression and its downstream gene Dt1 in the two parents, thereby affecting PH, NN, IL, and grain weight per plant without altering stem growth habit. Compared to the PH18(HXW) allele, the qPH18(JKK378) allele suppresses Dt2 expression, which releases the inhibition of Dt1 expression, thus enhancing NN and grain yield. Our findings shed light on the mechanism underlying NN and PH in soybean and provide a molecular marker to facilitate breeding.

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