CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice
文献类型： 外文期刊
作者： Lu, Qinghua 1 ; Luo, Xiangwen 1 ; Yang, Xiao 1 ; Zhou, Tong 3 ; Zhang, Yu 2 ; Lan, Ying 3 ; Zhang, Deyong 1 ; Zheng, Limin 2 ; Li, Yixin 1 ; Li, Li 1 ; Zhang, Songbai 1 ; Liu, Yong 1 ;
作者机构： 1.Hunan Univ, Longping Branch Biol Coll, Changsha, Peoples R China
2.Hunan Acad Agr Sci, State Key Lab Hybird Rice, Changsha, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety, Nanjing, Peoples R China
关键词： CRISP; Cas9; vacuolar ATPase subunit d; phytohormone biosynthesis; virus resistance; rice
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of OsV-ATPase d in rice were generated using the CRISPR/Cas9 system, and mutation of OsV-ATPase d did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that OsV-ATPase d is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of OsV-ATPase d significantly increased JA and ABA biosynthesis and resistance against Southern rice black-streaked dwarf virus (SRBSDV), but it decreased resistance against Rice stripe virus (RSV) in rice. The data presented in this study reveal that OsV-ATPase d mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.
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