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Disease resistance and genetic characteristics of genetically selected largemouth bass (Micropterus salmoides) revealed by whole genome resequencing

文献类型： 外文期刊

作者： Sun, Hui 1 ; Hua, Jixiang 1 ; Tao, Yifan 1 ; He, Jie 1 ; Wang, Qingchun 2 ; Dong, Yalun 2 ; He, Jixiang 3 ; Qiang, Jun 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China

3.Anhui Acad Agr Sci, Fisheries Res Inst, Hefei 230041, Peoples R China

关键词： Micropterus salmoides; LMBV; Whole genome resequencing; Genetic variation; Artificial selection

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2025 年 165 卷

页码：

收录情况： SCI

摘要： Largemouth bass ranavirus (LMBV) is a serious threat to aquacultured largemouth bass (Micropterus salmoides) because it is highly infectious and lethal. Our research group has carried out genetic improvement of largemouth bass with LMBV resistance as a target trait through family selection, combined with molecular marker-assisted selection, using the northern U.S.A. subspecies of largemouth bass as a foundation population. By 2023, we had generated 35 F2 generation LMBV-resistant families. The top six lines with the highest survival rates were selected through LMBV virus challenge experiments, leading to the construction of an F3-generation resistant population (SF) in 2024. After LMBV virus infection, the 7-day survival rate of the SF population was 28.9 % higher than that of the unselected control population (CF). Whole genome resequencing revealed higher genetic diversity of the SF population (PIC = 0.1557 vs. 0.1029, Ao = 1.8935 vs. 1.4329, Ho = 0.1647 vs. 0.1146) compared with the CF population. Forty-nine genomic regions selected in the SF population vs. the CF population were identified by selective scanning analysis (FST-ZHp/FST-theta pi), and 169 candidate genes were localized. Among them, the immune regulation-related genes atp7b, nampt, hpcal4, and ppsst1/2 were identified as key candidate genes for disease resistance under intensive selection in the SF population. These results provide new information about the genetic mechanism of LMBV resistance in largemouth bass, and theoretical support for further research on virus resistance and the selection of disease-resistant varieties. The identified genes and genomic regions are of great significance for the precision breeding of largemouth bass.

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