Second-order Calibration-assisted Excitation-emission Matrix Fluorescence Detection of Multi-class Mycotoxins in Grape, Wine and Raisin for Food Safety Assessment
文献类型： 外文期刊
作者： Shuangnan, Xie 1 ; Chao, Tang 1 ; Hongtao, Jia 1 ; Qi, Shen 3 ; Weizhong, He 3 ; Shiyi, Deng 4 ; Jing, Tang 4 ; Zhi, Liu 4 ; Cheng, Wang 5 ;
作者机构： 1.Xinjiang Agr Univ, Coll Resources & Environm, Urumqi 830052, Peoples R China
2.Xinjiang Agr Univ, Coll Life Sci, Xinjiang Key Lab Ecol Adaptat & Evolut Extreme Env, Urumqi 830052, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Agr Prod Qual & Safety Risk Assessment Lab Minist, Urumqi 830091, Peoples R China
4.Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi 417000, Peoples R China
5.Xinjiang Acad Agr Sci, Agr Prod Qual & Safety Risk Assessment Lab Minist, Urumqi 830091, Peoples R China
关键词： Grape product; Mycotoxin analysis; EEM detection; ATLD algorithm; Food safety
期刊名称：JOURNAL OF FLUORESCENCE （ 影响因子：3.1； 五年影响因子：2.9 ）
ISSN： 1053-0509
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Mycotoxin in grape and its products (wine, raisin) is a widely concerned issue of food safety, it closely associated with consumers' health. In this study, an analytical strategy by combining second-order calibration method with excitation-emission matrix (EEM) fluorescence detection followed photo derivatization (PD) was explored for rapid and sensitive analysis of aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZEA) in grape, raisin and wine. Except simple solvent extraction by ethyl acetate and concentration by vacuum distillation, samples don't need other complicated treatment steps any more. With the aid of predominant second-order advantages of alternative trilinear decomposition (ATLD) algorithm, 'pure' spectra and quantitative signals of targeted mycotoxins can be resolved from the heavily interfered EEM profile of sample even in the presence of spectral overlaps and unknown backgrounds. The recoveries of AFB1, OTA and ZEA in four kinds of samples are in the range of 90% to 110%, the limits of detection (LODs) were low to 0.1 mu g kg-1, 0.5 mu g kg-1 and 0.8 mu g kg-1, respectively. This analytical strategy may be as an alternative method for improving mycotoxin analysis in complex food matrices and ensuring food safety in grape industry.
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