您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A protoplast-based transient expression system for melon (Cucumis melo L.)

文献类型： 外文期刊

作者： Xu, Xinyang 1 ; Shen, Jia 1 ; Zhang, Yuejian 1 ; Niu, Xiaowei 1 ; Li, Guojing 1 ; Shou, Weisong 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Peoples R China

关键词： CmAPRR2; melon (Cucumis melo L; ); protoplast isolation; PEG- mediated transformation; subcellular localization

期刊名称：EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE （ 影响因子：1.074； 五年影响因子：1.641 ）

ISSN： 1611-4426

年卷期： 2021 年 86 卷 6 期

页码：

收录情况： SCI

摘要： Melon (Cucumis melo L.), an important horticultural and economic crop, is widely cultivated all over the world. Functional analysis of genes related to important agronomic traits will greatly promote the development of basic research in melon. The transient transformation system is a convenient method to analyze gene function in vivo and has been developed and applied in many species. Our objective was to develop an effective transformation system in melon. By comparing cotyledons and first true leaves, and balancing protoplast yield and protoplast vitality, optimal sampling tissue and enzymolysis time were obtained. Through analyzing effects of main parameters including PEG concentration, plasmid DNA amount and transfection time, on transformation efficiency, the optimal procedure for PEG-mediated transformation was obtained. Moreover, the subcellular localization of a GLK2-like transcription factor CmAPRR2 was analyzed by using this system. Results showed that first true leaves could yield more isolated protoplast than did cotyledons. The optimal time of enzymatic digestion is 6 h. The PEG concentration and plasmid DNA amount have a greater effect than incubation time on transformation efficiency. The peak transformaconcentration is 40% and 20 ig plasmid DNA is injected. In addition, subcellular localization showed that CmAPRR2 is a nuclear-localized protein. This research shows that the developed transient expression system based on protoplast isolation from first true leaves and PEG-mediated transformation is efficient and can be applied for functional gene analysis research in melon.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台