文献类型： 外文期刊

作者： Su, Xiang Ning ² ; Liu, Xue Song ¹ ; Li, Chuan Ying ² ; Zhang, Yu Ping ² ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Nanjing 210014, Peoples R China

2.Guangdong Acad Agr Sci, Res Inst Plant Protect, Guangzhou 510640, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Green Prevent & Control Fruits & Vegetable, Guangzhou 510640, Peoples R China

4.Key Lab High Technol Plant Protect Guangdong Prov, Guangzhou 510640, Peoples R China

关键词： cytochrome P450; isoproturon; acetochlor; rice; degradation; detoxification

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The rice cytochrome P450 gene has been comprehensively studied in the present study. This gene encodes CYP90D5 in promoting the degradation of isoproturon (IPU) and acetochlor (ACT) in rice tissues and grains. It has here been found that CYP90D5 improved the resistance of the plant to IPU and ACT, which was reflected in the improvement of the growth of the overexpression (OE) lines. CYP90D5 also reduced the levels of IPU and ACT accumulation in rice, and the CRISPR-Cas9 (Cas9) lines displayed the opposite effects. This function of CYP90D5 for pesticide degradation was also confirmed by the transformation of CYP90D5 in Pichia pastoris. Compared with the control yeast, it grew better and could degrade more pesticides. In addition, the relative contents of the IPU and ACT derivatives increased in the OE rice, while they decreased in the Cas9 rice. This suggested that CYP90D5 plays a pivotal role in the pesticide detoxification and degradation.