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Surface Functionalization of SBA-15 for Immobilization of Myoglobin

文献类型： 外文期刊

作者： Miao, Hengmin 1 ; Li, Maosheng 1 ; Wang, Fang 1 ; Li, Jiao 2 ; Lin, Ying-Wu 4 ; Xu, Jiakun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao, Peoples R China

2.Shandong Univ Technol, Sch Mat Sci & Engn, Zibo, Peoples R China

3.Qilu Univ Technol, Shandong Acad Sci, Sch Food Sci & Engn, Jinan, Peoples R China

4.Univ South China, Sch Chem & Chem Engn, Hengyang, Peoples R China

5.Univ South China, Key Lab Prot Struct & Funct Univ Hunan Prov, Hengyang, Peoples R China

关键词： myoglobin; SBA-15; organic functionalization; protein immobilization; biocatalysis

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：6.064； 五年影响因子：6.303 ）

ISSN： 2296-4185

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： Mesoporous molecular sieve SBA-15 was successfully modified with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The functionalized SBA-15 were characterized by small-angle X-ray (SAXRD), thermogravimetric analysis (TG), N-2 adsorption, and Fourier transformed infrared spectrum (FT-IR). APTES functionalized SBA-15 (named SBA-15-A) and GPTMS functionalized SBA-15 (named SBA-15-G) were used to immobilize myoglobin (Mb). The loading amounts of Mb by SBA-15-A and SBA-15-G were 511.2 and 547.8 mg/g, respectively, whereas only 359.6 mg/g was achieved by SBA-15. Mb/SBA-15-G and Mb/SBA-15-A demonstrated better reusability than SBA-15, retaining 84.6% and 82.7% of the initial activity after repeated use seven times. The Mb/SBA-15-A and Mb/SBA-15-G also exhibited improved thermal stability and storage stability.

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