Engineering high amylose and resistant starch in maize by CRISPR/Cas9-mediated editing of starch branching enzymes
文献类型： 外文期刊
作者： Ma, Mingzheng 1 ; Sun, Shanqiu 1 ; Zhu, Jinjie 1 ; Qi, Xiantao 1 ; Li, Gaoke 4 ; Hu, Jianguang 4 ; Xie, Chuanxiao 1 ; Liu, Changlin 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China
2.Hainan Yazhou Bay Seed Lab, Sanya 572024, Hainan, Peoples R China
3.Anhui Agr Univ, Hefei 230036, Anhui, Peoples R China
4.Guangdong Acad Agr Sci, Crops Res Inst, Guangzhou 510640, Guangdong, Peoples R China
关键词： Maize; Gene editing; Starch branching enzyme I; Starch branching enzyme IIb
期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）
ISSN： 2095-5421
年卷期： 2024 年 12 卷 4 期
页码：
收录情况： SCI
摘要： To improve the amylose content (AC) and resistant starch content (RSC) of maize kernel starch, we employed the CRISPR/Cas9 system to create mutants of starch branching enzyme I (SBEI) and starch branching enzyme IIb (SBEIIb). A frameshift mutation in SBEI (E1, a nucleotide insertion in exon 6) led to plants with higher RSC (1.07%), lower hundred-kernel weight (HKW, 24.71 +/- 0.14 g), and lower plant height (PH, 218.50 +/- 9.42 cm) compared to the wild type (WT). Like the WT, E1 kernel starch had irregular, polygonal shapes with sharp edges. A frameshift mutation in SBEIIb (E2, a four-nucleotide deletion in exon 8) led to higher AC (53.48%) and higher RSC (26.93%) than that for the WT. E2 kernel starch was significantly different from the WT regarding granule morphology, chain length distribution pattern, X-ray diffraction pattern, and thermal characteristics; the starch granules were more irregular in shape and comprised typical B-type crystals. Mutating SBEI and SBEIIb (E12) had a synergistic effect on RSC, HKW, PH, starch properties, and starch biosynthesis-associated gene expression. SBEIIa, SS1, SSIIa, SSIIIa, and SSIIIb were upregulated in E12 endosperm compared to WT endosperm. This study lays the foundation for rapidly improving the starch properties of elite maize lines.
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