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Development of a Gene-Based High Resolution Melting (HRM) Marker for Selecting the Gene ty-5 Conferring Resistance to Tomato Yellow Leaf Curl Virus

文献类型： 外文期刊

作者： Wang, Yinlei 1 ; Song, Liuxia 1 ; Zhao, Liping 1 ; Yu, Wengui 1 ; Zhao, Tongmin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Peoples R China

2.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

3.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China

关键词： tomato yellow leaf curl virus; ty-5 gene; HRM; functional marker

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 2 期

页码：

收录情况： SCI

摘要： Tomato yellow leaf curl virus (TYLCV) causes serious yield reductions in China. The use of certain resistance genes in tomato varieties has alleviated the impact of the virus to a certain extent. Recently, varieties with the Ty-1, Ty-2, or Ty-3 genes lost their resistance to TYLCV in some areas in China. New genes should be introduced into tomato to maintain the resistance to TYLCV. Tomato line AVTO1227 has excellent resistance to disease due to the resistance gene ty-5. In this study, we screened different types of markers in a tomato F-2 population to compare their accuracy and efficiency. The sequencing analysis results were consistent with the high resolution melting (HRM) marker genotype and field identification results. The result confirmed that the functional marker of ty-5 was accurate and reliable. The single nucleotide polymorphism-based HRM genotyping method established in this study can be used for the selection of breeding parent material, gene correlation analysis, and molecular marker-assisted breeding.

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