您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Diversity and Biocontrol Potential of Culturable Endophytic Fungi in Cotton

文献类型： 外文期刊

作者： Jin, Lirong 1 ; Yang, Long 1 ; Li, Wenjing 2 ; Xu, Dong 2 ; Yang, Nina 2 ; Li, Guoqing 1 ; Wan, Peng 2 ;

作者机构： 1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Key Lab Plant Pathol Hubei Prov, Wuhan, Peoples R China

2.Hubei Acad Agr Sci, Key Lab Integrated Pest Management Crops Cent Chi, Minist Agr, Wuhan, Peoples R China

3.Hubei Acad Agr Sci, Plant Protect Soil & Fertilizer Res Inst, Hubei Key Lab Crop Dis Insect Pests & Weeds Contr, Wuhan, Peoples R China

关键词： cotton; Verticillium dahliae; endophytic fungi; Fusarium proliferatum 10R-7; biological control

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Healthy cotton samples were collected and 93 endophytic fungal strains were isolated: 23 strains from the roots and 70 strains from the stems. Morphological characterization and ITS sequence analysis were used for the identification of these isolates. The results showed that the 93 strains including 20 species were highly diverse in terms of their taxonomy. Simpson's and Shannon's diversity indices were 0.915 and 3.848, respectively. Fusarium and Alternaria were the two dominant genera, constituting 19.4% of the total strains. Then, 72 spore-producing strains were tested for the suppression of cotton Verticillium wilt (CVW) caused by Verticillium dahliae in a greenhouse. Five strains exhibited effective suppression of CVW with average efficacy values higher than 50%. One of the effective strains, namely, Fusarium proliferatum 10R-7, was selected for the investigation of the role of fusaric acid, a secondary metabolite of strain 10R-7, in the suppression of V. dahliae and CVW. The results showed that F. proliferatum 10R-7 could produce fusaric acid, and this metabolite exhibited 100% inhibition of mycelial growth of V. dahliae at concentrations higher than 20 mu g/ml. However, fusaric acid at 2.5 to 80 mu g/ml was not effective in the suppression of CVW, compared with the control treatment with V. dahliae alone. F. proliferatum 10R-7 was labeled with green fluorescent protein (GFP), and the GFP-tagged strain was found to be able to colonize inside the taproots of cotton, suggesting that F. proliferatum 10R-7 is a true endophyte of cotton and endophytic colonization may play a role in the suppression of infection of cotton by V. dahliae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台