文献类型： 外文期刊

作者： Wang Siwei ¹ ; Zeng Guangfeng ² ; Liu Yanping ¹ ; Wang Xiaonan ¹ ; Sun Haibin ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Tecnol Plant Protect, Inst Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Inspect & Quarantine Tecnol Ctr, Guangzhou 510623, Guangdong, Peoples R China

关键词： high performance liquid chromatography (HPLC ); triple quadrupole mass spectrometry (MS/MS); glufosinate-ammonium; metabolites; litchi; banana

期刊名称：CHINESE JOURNAL OF CHROMATOGRAPHY （ 影响因子：0.7； 五年影响因子：0.5 ）

ISSN： 1000-8713

年卷期： 2019 年 37 卷 1 期

页码：

收录情况： SCI

摘要： A method was developed for the determination of glufosinate-ammonium and its metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry. The samples were ultrasound extracted by water, centrifuged to precipitate , and filtered through a 0.22 mu m Millipore membrane. The sample extract was separated by an Extend-C18 column with 0. 1% ammonia-methanol (9 :1 , v/v) as mobile phase with an isocratic elution , identified by electrospray ionization in negative and multiple reaction monitoring modes, and quantified with external standard method. Moreover, the pretreatment and mass spectrometry conditions were discussed. The method showed good linear relationship in the range of 1-1 000 mu g/L for glufosinate-ammonium and its metabolites. The average recoveries ranged from 82. 9% to 98. 6% with the relative standard deviations of 2. 6% -6. 3% when the litchi and banana samples were spiked at 50, 100, 1 000 mu g/kg. The limits of detection were 5-10 mu g/kg. The method has rapid analysis speed as well as high reliability and sensitivity, which are suitable for the fast determination of glufosinate-ammonium and its metabolites in litchi and banana samples.