您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Visual Detection of Chicken Adulteration Based on a Lateral Flow Strip-PCR Strategy

文献类型： 外文期刊

作者： Xu, Haoyi 1 ; Lan, Hangzhen 1 ; Pan, Daodong 2 ; Xu, Junfeng 4 ; Wang, Xiaofu 4 ;

作者机构： 1.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

2.Ningbo Univ, Key Lab Anim Prot Deep Proc Technol Zhejiang Prov, Ningbo 315800, Peoples R China

3.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315800, Peoples R China

4.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Traceabil Agr Genetically Modified Organi, Hangzhou 310021, Peoples R China

关键词： chicken; meat adulteration; DNA extraction; polymerase chain reaction; lateral flow strip

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2022 年 11 卷 15 期

页码：

收录情况： SCI

摘要： The aim of this study was to develop an accurate, easy-to-use, and cost-effective method for the detection of chicken adulteration based on polymerase chain reaction (PCR) and lateral flow strip (LFS). We compared six DNA extraction methods, namely the cetyltrimethylammonium bromide (CTAB) method, salt method, urea method, SDS method, guanidine isothiocyanate method, and commercial kit method. The chicken cytb gene was used as a target to design specific primers. The specificity and sensitivity of the PCR-LFS system were tested using a self-assembled lateral flow measurement sensor. The results showed that the DNA concentration obtained by salt methods is up to 533 +/- 84 ng mu L-1, is a suitable replacement for commercial kits. The PCR-LFS method exhibits high specificity at an annealing temperature of 62 degrees C and does not cross-react with other animal sources. This strategy is also highly sensitive, being able to detect 0.1% of chicken in artificial adulterated meat. The results of the test strips can be observed with the naked eye within 5 min, and this result is consistent with the electrophoresis result, demonstrating its high accuracy. Moreover, the detection system has already been successfully used to detect chicken in commercial samples. Hence, this PCR-LFS strategy provides a potential tool to verify the authenticity of chicken.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台