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Characterization of a membrane Fcγ receptor in largemouth bass (Micropterus saloumoides) and its response to bacterial challenge

文献类型： 外文期刊

作者： Wang, Jingya 1 ; Wu, Jing 1 ; Ma, Yanping 2 ; Hao, Le 2 ; Huang, Wen 4 ; Liu, Zhenxing 2 ; Li, Yugu 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China

3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China

4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

关键词： Membrane; Fc gamma R; Largemouth bass; Nocardia seriolae; Immune response

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Fc receptors (FcRs), specific to the Fc portion of immunoglobulin (Ig), are required to regulate immune responses against pathogenic infections. However, Fc gamma R is a member of FcRs family, whose structure and function remains to be elucidated in teleost fish. In this study, the Fc gamma RII, from largemouth bass (Micropterus saloumoides), named membrane MsFc gamma RII (mMsFc gamma RII), was cloned and identified. The opening reading frame (ORF) of mMsFc gamma RII was 750 bp, encoding 249 amino acids with a predicted molecular mass of 27 kDa. The mMsFc gamma RII contained a signal peptide, two Ig domains, a transmembrane domain, and an intracellular region, which was highly homology with Fc gamma R from other teleost fish. The mRNA expression analysis showed that mMsFc gamma RII was widely distributed in all tested tissues and with the highest expression level in spleen. After bacterial challenge, the expression of mMsFc gamma RII was significantly upregulated in vivo (spleen and head kidney), as well as in vitro (leukocytes from head kidney). The subcellular localization assay revealed that mMsFc gamma RII was mostly observed on the membrane of HEK293T cells which were transfected with mMsFc gamma RII overexpression plasmid. Flow cytometric analysis showed that natural mMsFc gamma RII protein was highly expressed in head kidney lymphocytes. Moreover, indirect immunofluorescence assay and pull-down assay indicated that mMsFc gamma RII could bind to IgM purified from largemouth bass serum. These results suggested that mMsFc gamma RII was likely to play an influential role in the immune response against pathogens and provided valuable insights for studying the function of FcRs in teleost.

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