Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology
文献类型： 外文期刊
作者： Xu, Cuihong 1 ; Zhong, Lingkun 1 ; Huang, Zeming 1 ; Li, Chenying 1 ; Lian, Jiazhang 2 ; Zheng, Xuefang 3 ; Liang, Yan 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China
2.Zhejiang Univ, Coll Chem & Biol Engn, Key Lab Biomass Chem Engn, Minist Educ, Hangzhou 310027, Peoples R China
3.Fujian Acad Agr Sci, Agr Bioresources Res Inst, 247 Wusi Rd, Fuzhou 350003, Peoples R China
关键词： Ralstonia solanacearum; Arabidopsis; Bioluminescence; LuxCDABE; Bacterial wilt
期刊名称：PLANT METHODS （ 影响因子：5.827； 五年影响因子：5.904 ）
ISSN：
年卷期： 2022 年 18 卷 1 期
页码：
收录情况： SCI
摘要： Background Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis-R. solanacearum system. However, the progress of R. solanacearum infection in Arabidopsis is still unclear. Results We generated a bioluminescent R. solanacearum by expressing plasmid-based luxCDABE. Expression of luxCDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 10(4) to 10(8) CFU center dot mL(-1). After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants. Conclusions Bioluminescent R. solanacearum strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of R. solanacearum-Arabidopsis interaction in the future.
- 相关文献
作者其他论文 更多>>
-
Metabolomic Profiling of Tomato Root Exudates Induced by Ralstonia solanacearum Strains of Different Pathogenicity: Screening for Metabolites Conferring Bacterial Wilt Resistance
作者：Chen, Zheng;Liu, Lianlian;Lin, Enquan;Liu, Bo;Zheng, Xuefang;Chen, Meichun;Lin, Xia;Li, Wangyu;Feng, Junjie
关键词：Bacterial wilt; biofilm formation; metabolome; pathogenicity; Ralstonia solanacearum; root exudates
-
Genome-wide identification and analysis of SmRR gene family in eggplant (Solanum melongena L.) and their response to abiotic stress and auxin
作者：Li, Wenjing;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Wu, Weiren;Zhou, Yuanchang;Zheng, Yan;Li, Wenjing;Zhou, Yuanchang;Zheng, Xuefang;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Zheng, Yan;Li, Wenjing;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Wu, Weiren;Zhou, Yuanchang;Zheng, Yan
关键词：Eggplant; RR gene family; Genome-wide identification; Abiotic stress; IAA treatment
-
Testing a biocontrol agent consortium for suppression of tomato bacterial wilt through rhizosphere microecological regulation
作者：Zheng, Xuefang;Wang, Jieping;Chen, Meichun;Chen, Yanping;Chen, Zheng;Liu, Bo;Wang, Mingkuang
关键词：Bacterial wilt; Biocontrol control agent consortium; Microecological regulation; Ralstonia solanacearum; Rhizosphere
-
Bacillus lipopeptides inhibit lipase activity and promote 3T3-L1 preadipocyte differentiation
作者：Chen, Meichun;Chen, Deju;Xiao, Rongfeng;Zheng, Xuefang;Liu, Bo;Wang, Jieping
关键词：Lipopeptide; lipase inhibitory activity; PPAR gamma
-
Genetic stability of virulent, intermediate, and avirulent strains of Ralstonia solanacearum after extensive, consecutive subculturing
作者：Zheng, Xuefang;Chen, Meichun;Chen, Zheng;Wang, Jieping;Zhu, Yujing;Wang, Ziran
关键词：Consecutive subculture; Genetic stability; Pathogenicity; Ralstonia solanacearum
-
An avirulent Ralstonia solanacearum strain FJAT1458 outcompetes with virulent strain and induces tomato plant resistance against bacterial wilt
作者：Zheng, Xuefang;Xiao, Rongfeng;Chen, Meichun;Wang, Jieping;Wu, Huijun;Gao, Xuewen
关键词：bacterial wilt; colonization; defense related gene; Ralsonia solanacearum; red fluorescent protein
-
Bacillus dafuensis sp. Nov., Isolated from a Forest Soil in China
作者：Zheng, Xuefang;Wang, Jieping;Zhang, Haifeng;Liu, Bo;Wang, Ziran
关键词：
