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An avirulent Ralstonia solanacearum strain FJAT1458 outcompetes with virulent strain and induces tomato plant resistance against bacterial wilt

文献类型： 外文期刊

作者： Zheng, Xuefang 1 ; Xiao, Rongfeng 1 ; Chen, Meichun 1 ; Wu, Huijun 2 ; Gao, Xuewen 2 ; Wang, Jieping 1 ;

作者机构： 1.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Peoples R China

2.Nanjing Agr Univ, Key Lab Integrated & Management Crop Dis & Pests, Minist Educ, Dept Plant Pathol,Coll Plant Protect, Nanjing 210095, Peoples R China

关键词： bacterial wilt; colonization; defense related gene; Ralsonia solanacearum; red fluorescent protein

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期： 2022 年 78 卷 11 期

页码：

收录情况： SCI

摘要： BACKGROUND Bacterial wilt (BW) caused by Ralstonia solanacearum (RS) is considered as one of the most destructive plant diseases. An avirulent strain of RS, FJAT1458, is a potential biocontrol agent of BW. In this study, the mechanism of FJAT1458 against BW was evaluated. RESULTS FJAT1458 was tagged with the red fluorescent protein gene, and the resulting strain was named as FJAT1458-RFP. When FJAT1458-RFP and FJAT91-GFP (a virulent strain of RS labelled with the green fluorescent protein gene), were co-inoculated in potted tomato plants, the colonization of FJAT91-GFP reached an almost undetectable level at 7 days post-inoculation (dpi) in the roots and at 9 dpi in rhizosphere soil. When they were co-inoculated in a hydroponic tomato growing system, numbers of the two strains were similar at 3 dpi in the root tissues; however, FJAT91-GFP was not detected at 9 dpi while FJAT1458-RFP maintained 1.77 x 10(5) CFU g(-1). The inoculation of FJAT1458-RFP alone or combination with FJAT91-GFP significantly increased tomato root activity. Moreover, expression levels of the defense-related genes PR-1a, GLUA, and CHI3 in tomato roots were significantly up-regulated by FJAT1458-RFP and co-inoculation of FJAT1458-RFP and FJAT91-GFP at 5 dpi, compared to the control (water, CK) treatment. Noteworthy, expression levels of GLUA in the treatments of FJAT1458-RFP and FJAT1458-RFP + FJAT91-GFP were 12.22- and 12.05-fold higher than that in the CK at 5 dpi, respectively. CONCLUSIONS The results suggested that the avirulent strain FJAT1458-RFP could suppress colonization of the virulent strain in tomato roots, and induce tomato plant resistance against BW. (c) 2022 Society of Chemical Industry.

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