Lipopeptides from Bacillus velezensis induced apoptosis-like cell death in the pathogenic fungus Fusarium concentricum
文献类型： 外文期刊
作者： Chen, Meichun 1 ; Deng, Yingjie 1 ; Zheng, Meixia 4 ; Xiao, Rongfeng 1 ; Wang, Xun 2 ; Liu, Bo 1 ; He, Jin 2 ; Wang, Jieping 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Resources Environm & Soil Fertilizer, Fuzhou 350003, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430000, Peoples R China
3.Huazhong Agr Univ, Hubei Hongshan Lab, Wuhan 430000, Peoples R China
4.Fujian Acad Agr Sci, Inst Crop Sci, Fuzhou 350003, Peoples R China
关键词： crop protection; antifungal peptides; lipopeptides; pathogenic fungus; Fusarium concentricum; cell apoptosis
期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：4.3 ）
ISSN： 1364-5072
年卷期： 2024 年 135 卷 3 期
页码：
收录情况： SCI
摘要： Aims Stem rot caused by Fusarium concentricum is a new disease of Paris polyphylla reported by our research group. The present study investigates the growth inhibitory and apoptotic effects of Bacillus velezensis FJAT-54560 lipopeptide against F. concentricum.Methods and Results HPLC preparation and LC-MS analysis results show that the crude lipopeptides secreted by Bacillus velezensis FJAT-54560 isolated from Jasminum sambac consist of C14-17 iturin A, C14 fengycin B, C16 fengycin A/A2, C18 fengycin A, C20 fengycin B2, C21 fengycin A2, C22-23 fengycin A, C12-16 surfactin A, and C15 surfactin A derivatives. The mass ratios (g/g) of iturin, fengycin, and surfactin in lipopeptides are 2.40, 67.51, and 30.08%, respectively. Through inhibition zone and inhibition rate experiments, we found that crude lipopeptides and purified fengycin exhibit strong antifungal activity against F. concentricum, including accumulation of reactive oxygen species, loss of mitochondrial membrane potential, DNA fragmentation, Ca2+ accumulation, chromatin condensation, and phosphatidylserine externalization. Transcriptomic analysis indicates that crude lipopeptide-induced apoptosis in F. concentricum cells may be mediated by apoptosis-inducing factors and apoptosis mediators and can serve as a metacaspase-independent model.Conclusion Lipopeptides from Bacillus velezensis FJAT-54560 can control the pathogenic fungus F. concentricum by inducing apoptosis.
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