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Production of Mycelial Biomass and Exo-polymer by Hericium erinaceus CZ-2: Optimization of Nutrients Levels using Response Surface Methodology

文献类型： 外文期刊

作者： Cui, Fengjie 1 ; Liu, Zhiqiang 3 ; Li, Yin 5 ; Ping, Lifeng 6 ; Ping, Liying 3 ; Zhang, Zhicai 1 ; Lin, Lin 1 ; Dong, Yin 1 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biotechnol, Zhenjiang 212013, Peoples R China

2.Ohio State Univ, Dept Food Agr & Biol Engn, Wooster, OH 44691 USA

3.Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China

4.NYU, Polytech Univ, Dept Chem & Biol Sci, NSF UCRC Ctr Biocatalysis & Bioproc Macromol 1, Brooklyn, NY 11201 USA

5.N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA

6.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

关键词： hericium erinaceus;doehlert design;submerged culture;medium optimization;biomass;exo-polymer

期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： The Doehlert experimental design was used to optimize the production of mycelial biomass and exo-polymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract, and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose; 1 g/L, KH_2PO_4; and 0.5 g/L, MgSO_4·7H_2O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour; 2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH_2PO_4; and 0.5 g/L, MgSO_4·7H_2O. The upscale production was also investigated using a 15 L fermentor using the optimized medium.

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