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Molecular cloning and mRNA expression of peroxiredoxin gene in black tiger shrimp (Penaeus monodon)

文献类型： 外文期刊

作者： Qiu, Lihua 1 ; Ma, Zhuojun 2 ; Jiang, Shigui 1 ; Wang, Weifang 1 ; Zhou, Falin 1 ; Huang, Jianhua 1 ; Li, Jianzhu 1 ; Yan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Biotechnol & Aquiculture Lab, Guangzhou 510300, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100039, Peoples R China

关键词： Cloning;RT expression;Black tiger shrimp (Penaeus monodon);Internet resource.

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The techniques of homology cloning and anchored PCR were used to clone the peroxiredoxin (Prx) gene from black tiger shrimp (Penaeus monodon). The full length cDNA of black tiger shrimp Prx (PmPrx) contained a 5o untranslated region (UTR) of 51 bp, an ORF (open reading frame) of 582 bp encoding a polypeptide of 193 amino acids with an estimated molecular mass of 22.15 kDa and a 3o UTR of 948 bp. Sequence comparison showed that PmPrx shared higher identities with Prx IVs than that with other isoforms of Prx, indicating PmPrx was a member of the Prx IV family. A quantitative reverse transcriptase Real-Time PCR (qRT-PCR) assay was developed to assess the mRNA expression of PmPrx in different tissues and the temporal expression of PmPrx in the hepatopancreas challenged by lipopolyssacharide (LPS). Higher-level mRNA expression of PmPrx was detected in the tissues of hepatopancreas, gonad and heart. The expression of PmPrx in the hepatopancreas was up regulated after stimulated by LPS. The results indicated that PmPrx was a constitutive and inducible expressed protein and could be induced by LPS.

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