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GENERATION AND CHARACTERIZATION OF 11 NOVEL EST DERIVED MICROSATELLITES FROM VICIA FABA (FABACEAE)

文献类型： 外文期刊

作者： Gong, Ya-Ming 1 ; Xu, Sheng-Chun 1 ; Mao, Wei-Hua 2 ; Hu, Qi-Zan 1 ; Zhang, Gu-Wen 1 ; Ding, Ju 1 ; Li, Ze-Yun 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310029, Zhejiang, Peoples R China

关键词： heterozygosity;genetic diversity;NCBI database;resource conservation

期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Premise of the study: Simple sequence repeat (SSR) markers were developed for faba bean using expressed sequence tags (ESTs) from the NCBI database to study for genetic diversity.Methods and Results: A total of 11 novel EST-SSR loci were generated and characterized when tested on four populations of 29 faba bean individuals from China and Europe. The number of alleles (A) ranged from 1 to 3 in each population, and observed heterozygosity (H-O) and expected heterozygosity (H-E) ranged from 0 to 0.5000 and 0.6400, respectively. Furthermore, transferable analysis revealed that eight of these loci (72.73%) amplified in Pisum sativum L., six of which (75.00%) detected polymorphism.Conclusions: The developed markers in this study will provide valuable tools for genetic diversity, resource conservation, genetic mapping, and marker-assisted breeding of faba bean in the future.

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