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A cassava common mosaic virus vector for virus-induced gene silencing in cassava

文献类型： 外文期刊

作者： Tuo, Decai 1 ; Zhou, Peng 1 ; Yan, Pu 1 ; Cui, Hongguang 5 ; Liu, Yang 1 ; Wang, He 1 ; Yang, Xiukun 1 ; Liao, Wenbin 1 ; Su 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Hainan Key Lab Protect & Utilizat Trop Bioresourc, Haikou 571101, Hainan, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Trop Agr Resources, Haikou 571101, Hainan, Peoples R China

5.Hainan Univ, Coll Plant Protect, Haikou 570228, Hainan, Peoples R China

6.Hainan Univ, Coll Hort, Haikou 570228, Hainan, Peoples R China

关键词： Cassava; Cassava common mosaic virus; Virus vector; Virus-induced gene silencing

期刊名称：PLANT METHODS （ 影响因子：4.993； 五年影响因子：5.312 ）

ISSN：

年卷期： 2021 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.

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