Transcription factors TgbHLH95 and TgbZIP44 cotarget terpene biosynthesis gene TgGPPS in Torreya grandis nuts
文献类型： 外文期刊
作者： Zhang, Zuying 1 ; Tao, Liu 1 ; Gao, Lingling 1 ; Gao, Yadi 1 ; Suo, Jinwei 1 ; Yu, Weiyu 1 ; Hu, Yuanyuan 1 ; Wei, Chunyan 3 ; Farag, Mohamed A. 4 ; Wu, Jiasheng 1 ; Song, Lili 1 ;
作者机构： 1.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Linan 311300, Zhejiang, Peoples R China
2.Zhejiang A&F Univ, Zhejiang Prov Key Lab Forest Aromat Plants Based H, Linan 311300, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Hort, Desheng Middle Rd 298, Hangzhou 310021, Zhejiang, Peoples R China
4.Cairo Univ, Coll Pharm, Pharmacognosy Dept, Kasr el Aini st, Cairo 11562, Egypt
期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）
ISSN： 0032-0889
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Terpenes are volatile compounds responsible for aroma and the postharvest quality of commercially important xiangfei (Torreya grandis) nuts, and there is interest in understanding the regulation of their biosynthesis. Here, a transcriptomics analysis of xiangfei nuts after harvest identified 156 genes associated with the terpenoid metabolic pathway. A geranyl diphosphate (GPP) synthase (TgGPPS) involved in production of the monoterpene precursor GPP was targeted for functional characterization, and its transcript levels positively correlated with terpene levels. Furthermore, transient overexpression of TgGPPS in tobacco (Nicotiana tabacum) leaves or tomato (Solanum lycopersicum) fruit led to monoterpene accumulation. Analysis of differentially expressed transcription factors identified one basic helix-loop-helix protein (TgbHLH95) and one basic leucine zipper protein (TgbZIP44) as potential TgGPPS regulators. TgbHLH95 showed significant transactivation of the TgGPPS promoter, and its transient overexpression in tobacco leaves led to monoterpene accumulation, whereas TgbZIP44 directly bound to an ACGT-containing element in the TgGPPS promoter, as determined by yeast 1-hybrid test and electrophoretic mobility shift assay. Bimolecular fluorescence complementation, firefly luciferase complementation imaging, co-immunoprecipitation, and GST pull-down assays confirmed a direct protein-protein interaction between TgbHLH95 and TgbZIP44 in vivo and in vitro, and in combination these proteins induced the TgGPPS promoter up to 4.7-fold in transactivation assays. These results indicate that a TgbHLH95/TgbZIP44 complex activates the TgGPPS promoter and upregulates terpene biosynthesis in xiangfei nuts after harvest, thereby contributing to its aroma. A bHLH-bZIP transcription complex regulates terpene biosynthesis by activating TgGPPS expression in Torreya grandis nuts during the postharvest ripening stage
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