Target Screening and Validation of the Antitumor Effect of Saponin Extract From Holothuria leucospilota
文献类型： 外文期刊
作者： Xu, Fuju 1 ; Liu, Guangchun 1 ; Zhao, Chujie 1 ; Zhang, Shenglin 1 ; Lin, Ruoyan 1 ; Cao, Xudong 2 ; Yu, Zonghe 3 ; Luo, Peng 4 ; Huang, Wen 5 ; Yuan, Lihong 1 ;
作者机构： 1.Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou, Peoples R China
2.Univ Ottawa, Dept Chem & Biol Engn, Ottawa, ON, Canada
3.South China Agr Univ, Coll Marine Sci, Guangzhou, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Peoples R China
5.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Anim Nutr & Feed Sci South China, Collaborat Innovat Ctr Aquat Sci,Inst Anim Sci,Gua, Guangzhou, Peoples R China
关键词：
antitumor activity; ESI-MS/MS;
期刊名称：CHEMISTRY & BIODIVERSITY （ 影响因子：2.5； 五年影响因子：2.6 ）
ISSN： 1612-1872
年卷期： 2025 年
页码：
收录情况： SCI
摘要： This paper aimed to isolate and characterize the saponins from Holothuria leucospilota and investigate their potential antitumor effects and antitumor targets using network pharmacology and bioinformatics. The purified saponins were analyzed using electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Ten saponins were identified. The in vitro experiments demonstrated that H. leucospilota saponins exhibited significant dose-dependent inhibition on HepG2, Panc02, and UM-UC-3 cell proliferation and differentiation. In addition, through network pharmacology, bioinformatics, and molecular docking, a total of 22 key tumor targets of H. leucospilota saponins were identified. Then, the reverse-transcription quantitative polymerase chain reaction (RT-qPCR) results showed that the saponins from H. leucospilota can inhibit the expression of target genes AURKB, BIRC5, CHEK1, PTGS2, and MMP9, and may affect the neuroactive ligand-receptor signaling pathway, thereby suppressing tumor proliferation and migration. In conclusion, we have demonstrated that the saponin extract from H. leucospilota has potential application value in tumor therapy.
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