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Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago

文献类型： 外文期刊

作者： Liu, Zhijian 1 ; Yang, Jun 2 ; Long, Yanping 1 ; Zhang, Chi 2 ; Wang, Dapeng 2 ; Zhang, Xiaowei 2 ; Dong, Wentao 2 ; Zhao, Li 4 ; Liu, Chengwu 4 ; Zhai, Jixian 1 ; Wang, Ertao 2 ;

作者机构： 1.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China

2.Chinese Acad Sci, Inst Plant Physiol & Ecol, Ctr Excellence Mol Plant Sci, New Cornerstone Sci Lab,Natl Key Lab Plant Mol Gen, Shanghai, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Treats Qual & S, Key Lab Biotechnol Plant Protect MOA China & Zheji, Hangzhou, Peoples R China

4.Univ Sci & Technol China, Sch Life Sci, Div Life Sci & Med, MOE Key Lab Membraneless Organelles & Cellular Dyn, Hefei, Peoples R China

5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2023 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Establishing legume-rhizobial symbiosis requires precise coordination of complex responses in a time- and cell type-specific manner. Encountering Rhizobium, rapid changes of gene expression levels in host plants occur in the first few hours, which prepare the plants to turn off defence and form a symbiotic relationship with the microbes. Here, we applied single-nucleus RNA sequencing to characterize the roots of Medicago truncatula at 30 min, 6 h and 24 h after nod factor treatment. We found drastic global gene expression reprogramming at 30 min in the epidermis and cortex and most of these changes were restored at 6 h. Moreover, plant defence response genes are activated at 30 min and subsequently suppressed at 6 h in non-meristem cells. Only in the cortical cells but not in other cell types, we found the flavonoid synthase genes required to recruit rhizobia are highly expressed 30 min after inoculation with nod factors. A gene module enriched for symbiotic nitrogen fixation genes showed that MtFER (MtFERONIA) and LYK3 (LysM domain receptor-like kinase 3) share similar responses to symbiotic signals. We further found that MtFER can be phosphorylated by LYK3 and it participates in rhizobial symbiosis. Our results expand our understanding of dynamic spatiotemporal symbiotic responses at the single-cell level.

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