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Biofortified Rice Provides Rich Sakuranetin in Endosperm

文献类型： 外文期刊

作者： Zhao, Yao 1 ; Hu, Jitao 1 ; Zhou, Zhongjing 3 ; Li, Linying 1 ; Zhang, Xueying 1 ; He, Yuqing 1 ; Zhang, Chi 1 ; Wang, Junmin 4 ; Hong, Gaojie 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Ke Lab Biotechnol Plant Protect, MOA China, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Cent Lab, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Crops & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Biofortified rice; Sakuranetin biosynthesis; Metabolic engineering; MALDI-MS imaging; Rice endosperm

期刊名称：RICE （ 影响因子：5.5； 五年影响因子：5.8 ）

ISSN： 1939-8425

年卷期： 2024 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Sakuranetin plays a key role as a phytoalexin in plant resistance to biotic and abiotic stresses, and possesses diverse health-promoting benefits. However, mature rice seeds do not contain detectable levels of sakuranetin. In the present study, a transgenic rice plant was developed in which the promoter of an endosperm-specific glutelin gene OsGluD-1 drives the expression of a specific enzyme naringenin 7-O-methyltransferase (NOMT) for sakuranetin biosynthesis. The presence of naringenin, which serves as the biosynthetic precursor of sakuranetin made this modification feasible in theory. Liquid chromatography tandem mass spectrometry (LC-MS/MS) validated that the seeds of transgenic rice accumulated remarkable sakuranetin at the mature stage, and higher at the filling stage. In addition, the panicle blast resistance of transgenic rice was significantly higher than that of the wild type. Specially, the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging was performed to detect the content and spatial distribution of sakuranetin and other nutritional metabolites in transgenic rice seeds. Notably, this genetic modification also did not change the nutritional and quality indicators such as soluble sugars, total amino acids, total flavonoids, amylose, total protein, and free amino acid content in rice. Meanwhile, the phenotypes of the transgenic plant during the whole growth and developmental periods and agricultural traits such as grain width, grain length, and 1000-grain weight exhibited no significant differences from the wild type. Collectively, the study provides a conceptual advance on cultivating sakuranetin-rich biofortified rice by metabolic engineering. This new breeding idea may not only enhance the disease resistance of cereal crop seeds but also improve the nutritional value of grains for human health benefits.

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