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Integrated physiological, transcriptome-wide m6A methylation and proteome analysis reveals molecular mechanisms of tomato root response to cadmium stress

文献类型： 外文期刊

作者： Liu, Chaochao 2 ; Zhao, Yao 2 ; Wen, Lang 2 ; Li, Zixing 2 ; Luo, Shaodan 2 ; Cheng, Yuan 1 ; Ahammed, Golam Jalal 4 ;

作者机构： 1.Xianghu Lab, Hangzhou 311231, Zhejiang, Peoples R China

2.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212100, Jiangsu, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Vegetables, State Key Lab Breeding Base Zhejiang Sustainable P, Hangzhou 310021, Zhejiang, Peoples R China

4.Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang 471023, Henan, Peoples R China

5.Henan Int Joint Lab Stress Resistance Regulat & Sa, Luoyang 471023, Henan, Peoples R China

关键词： Tomato; N6-methyladenosine (m6A) modification; Transcriptome; Proteome; Metal transporters

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）

ISSN： 2095-9885

年卷期： 2025 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Soil cadmium pollution has increasingly become a serious problem for crop production, which drastically attenuates plant growth and food safety. Although N6-methyladenosine (m6A) methylation is crucial for plant response to various stresses, the regulatory mechanism underlying m6A modification during cadmium (Cd) stress remains unclear. This study investigated the physiological responses, transcriptome-wide m6A methylome, and proteome changes in tomato roots exposed to 50 mmol $ L-1 CdCl2. Excess Cd restricted plant growth, altered the antioxidant system and disrupted mineral nutrient absorption. We identified a negative correlation between m6A levels and gene transcription for that 150 out of 198 differentially expressed genes (DEGs) were hypomethylated but mRNA up-regulated. Cd stress also enhanced translational efficiency, particularly for differentially abundant proteins (DAPs). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that differentially m6A modified genes (DMGs), DEGs, and DAPs were commonly enriched in phenylpropanoid biosynthesis, glutathione metabolism, and ABC transporters, reflecting cell wall barriers, chelation, and transport of Cd, respectively. Finally, we confirmed the Cd-transport activity of eight putative metal transporters identified in DMGs, DEGs, or DAPs by yeast complementaion experiments, and pharmacologically investigated the effect of m6A modification on their expression. Treatment with the m6A methylation inhibitor 3-deazaneplanocin A (3-DA) reduced SlIRT1/2 expression and increased SlNRAMP3/SlZIP4 expression, while the m6A demethylase inhibitor meclofenamic acid (MA) treatment decreased SlNRAMP3 expression but elevated SlIRT2 expression under Cd stress. Our findings provide novel insights into the interplay between m6A modification, transcription, and translation under Cd stress and the associated plant stress response.

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