文献类型： 外文期刊

作者： Zhang Zuofa ¹ ; Song Tingting ¹ ; Cai Weiming ¹ ; Wang Fugen ² ; Lv Guoying ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

2.Hangzhou Qiandao Xiugu Biotechnol Co Ltd, Hangzhou 311701, Zhejiang, Peoples R China

关键词： Polysaccharide; Sparassis latifolia; Depolymerisation methods; Antioxidant activity

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2021 年 110 卷

页码：

收录情况： SCI

摘要： Polysaccharides (SLP) derived from Sparassis latifolia were depolymerised by ultrasonication, hydrochloric acid, and H2O2/Vc to obtain the depolymerised polysaccharides U-SLP, H-SLP, and HV-SLP, respectively. Results from X-ray powder diffraction, atomic force microscopy, Fourier-transfrom infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and scanning electron microscopy indicated that the SLP were damaged to varying degrees by the three methods. The results showed that the three depolymerisation methods broke down the molecular chains, impeded aggregation, and reduced the molecular weight of the polysaccharides without changing their primary structure and monosaccharide composition of SLP. Relative to the original SLP, the depolymerised SLPs showed higher antioxidant activity during in vitro ABTS and reducing power assays. Also, the depolymerised SLPs could effectively improve the survival rate of zebrafish and reduce their heart rate, reactive oxygen species production, and cell death. The HV-SLP had the highest antioxidant activity of the three depolymerised fractions. The results revealed that the H2O2/Vc treatment is an effective method for preparing depolymerised SLP products with low molecular weights and dramatic antioxidant activity.