文献类型： 外文期刊

作者： Jin, Jie ¹ ; Wang, Jianfeng ² ; Li, Keke ¹ ; Wang, Shengwang ¹ ; Qin, Juan ¹ ; Zhang, Guohong ⁴ ; Na, Xiaofan ¹ ; Wang, Xiaomin ¹ ; Bi, Yurong ¹ ;

作者机构： 1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R China

2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China

3.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Ctr Grassland Microbiome, Collaborat Innovat Ctr Western Ecol Safety,State, Lanzhou 730000, Peoples R China

4.Gansu Acad Agr Sci, Inst Dryland Agr, Lanzhou 730070, Peoples R China

关键词： transcriptome; metabolome; amino acid; nitrogen metabolism; nitrogen use efficiency; TCA cycle; salt stress; soybean

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2021 年 22 卷 23 期

页码：

收录情况： SCI

摘要： Salinity stress is a threat to yield in many crops, including soybean (Glycine max L.). In this study, three soybean cultivars (JD19, LH3, and LD2) with different salt resistance were used to analyze salt tolerance mechanisms using physiology, transcriptomic, metabolomic, and bioinformatic methods. Physiological studies showed that salt-tolerant cultivars JD19 and LH3 had less root growth inhibition, higher antioxidant enzyme activities, lower ROS accumulation, and lower Na+ and Cl- contents than salt-susceptible cultivar LD2 under 100 mM NaCl treatment. Comparative transcriptome analysis showed that compared with LD2, salt stress increased the expression of antioxidant metabolism, stress response metabolism, glycine, serine and threonine metabolism, auxin response protein, transcription, and translation-related genes in JD19 and LH3. The comparison of metabolite profiles indicated that amino acid metabolism and the TCA cycle were important metabolic pathways of soybean in response to salt stress. In the further validation analysis of the above two pathways, it was found that compared with LD2, JD19, and LH3 had higher nitrogen absorption and assimilation rate, more amino acid accumulation, and faster TCA cycle activity under salt stress, which helped them better adapt to salt stress. Taken together, this study provides valuable information for better understanding the molecular mechanism underlying salt tolerance of soybean and also proposes new ideas and methods for cultivating stress-tolerant soybean.