文献类型： 外文期刊

作者： Li, Man-Wah ¹ ; Wang, Xin ¹ ; Sze, Ching-Ching ¹ ; Yung, Wai-Shing ¹ ; Wong, Fuk-Ling ¹ ; Zhang, Guohong ⁵ ; Chung, Gyuhwa ⁶ ; Chan, Ting-Fung ¹ ; Lam, Hon-Ming ¹ ;

作者机构： 1.Chinese Univ Hong Kong, Ctr Soybean Res, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China

2.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China

3.Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518000, Peoples R China

4.Guangzhou Univ, Sch Life Sci, Innovat Ctr Mol Genet & Evolut, Guangdong Prov Key Lab Plant Adaptat & Mol Design, Guangzhou 510006, Peoples R China

5.Gansu Acad Agr Sci, Inst Dryland Agr, Lanzhou 730030, Peoples R China

6.Chonnam Natl Univ, Dept Biotechnol, Yeosu 59626, South Korea

关键词： Glycine max; single nucleotide polymorphism (SNP); trait-specific marker; present-absent variation; tetra-primer ARMS-PCR; genotyping; breeding

期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN：

年卷期： 2023 年 13 卷 11 期

页码：

收录情况： SCI

摘要： Soybean (Glycine max L.) is gaining in importance due to its many uses, including as a food crop and a source of industrial products, among others. Increasing efforts are made to accelerate soybean research and develop new soybean varieties to meet global demands. Soybean research, breeding, identification, and variety protection all rely on precise genomic information. While DNA markers are invaluable tools for these purposes, the older generations, especially those developed before the advent of genome sequencing, lack precision and specificity. Thankfully, advancements in genome sequencing technologies have generated vast amounts of sequence data over the past decade, allowing precise and high-resolution analyses. However, making sense of the genomic information requires a certain level of professional training and computational power, which are not universally available to researchers. To address this, we generated a set of PCR-based DNA markers out of the existing genomic data from 228 popular soybean varieties that offer precise, unambiguous genomic information and can be easily adapted in various applications. A standard operating procedure (SOP) was also designed for these markers and validated on diverse soybean varieties to ensure their reproducibility. This user-friendly universal panel of DNA markers, along with the SOP, will facilitate soybean research and breeding programs through simple applications.