Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
文献类型： 外文期刊
作者： Xian, Jinwen 1 ; Zhao, Pengpeng 1 ; Wang, Ning 1 ; Wang, Weiye 1 ; Zhang, Yanyan 1 ; Meng, Jimeng 1 ; Ma, Xun 2 ; Wang, Z 1 ;
作者机构： 1.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Med, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China
2.Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Peoples R China
关键词： Echinococcus granulosus; cystic echinococcosis; Eg-TSP11; immunogenicity; dog vaccine
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）
ISSN：
年卷期： 2021 年 8 卷
页码：
收录情况： SCI
摘要： Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage of Echinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine development or disease diagnosis. In the present study, the major antigen coding sequence of tetraspanin 11 (Eg-TSP11) from E. granulosus was determined. The results of immunolocalization showed that Eg-TSP11 was mainly located in the tegument of adult worms and protoscoleces. Western blotting analysis showed that the serum from dogs injected with recombinant Eg-TSP11 (rEg-TSP11) could recognize Eg-TSP11 among natural protoscolex proteins. Moreover, the serum from dogs with E. granulosus infection also recognized rEg-TSP11. Serum indirect enzyme-linked immunosorbent assays demonstrated that IgG levels gradually increased after the first immunization with rEg-TSP11 compared with those in the control group. Furthermore, the serum levels of interleukin 4, interleukin 5, and interferon gamma were significantly altered in the rEg-TSP11 group. Importantly, we found that vaccination with rEg-TSP11 significantly decreased worm burden and inhibited segment development in a dog model of E. granulosus infection. Based on these findings, we speculated that rEg-TSP11 might be a potential candidate vaccine antigen against E. granulosus infection in dogs.
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