Expression and Functional Analysis of Two Cytochrome P450 Monooxygenase Genes and a UDP-Glycosyltransferase Gene Linked with Thiamethoxam Resistance in the Colorado Potato Beetle
文献类型： 外文期刊
作者： Wang, Yaqi 1 ; Tian, Yitong 2 ; Zhou, Dongdi 1 ; Fang, Jiayi 1 ; Cao, Jingwei 1 ; Shi, Chengcheng 1 ; Lei, Yixuan 1 ; Fu, Kaiyun 3 ; Guo, Wenchao 3 ; Jiang, Weihua 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Agr,Minist Educ,Dept Entomol,Key Lab Integr, Nanjing 210095, Peoples R China
2.Minist Agr & Rural Affairs Peoples Republ China, China State Farms Ecn Dev Ctr, South Subtrop Crops Ctr, Beijing 100122, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Plant Protect, Xinjiang Key Lab Agr Biosafety, Minist Agr,Key Lab Integrated Pest Management Crop, Urumqi 830091, Peoples R China
关键词： Leptinotarsa decemlineata; thiamethoxam; resistance; cytochrome P450; UDP-glycosyltransferase; RNA interference
期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）
ISSN：
年卷期： 2024 年 15 卷 8 期
页码：
收录情况： SCI
摘要： Cytochrome P450 monooxygenases (P450s) and UDP-glycosyltransferases (UGTs) are involved in the evolution of insecticide resistance. Leptinotarsa decemlineata (Say), the Colorado potato beetle (CPB), is a notorious insect that has developed resistance to various insecticides including neonicotinoids. This study investigated whether the differentially expressed P450 genes CYP9Z140 and CYP9AY1 and UGT gene UGT321AP1, found in our transcriptome results, conferred resistance to thiamethoxam in L. decemlineata. Resistance monitoring showed that the sampled field populations of L. decemlineata adults collected from Urumqi City and Qapqal, Jimsar, and Mulei Counties of Xinjiang in 2021-2023 developed low levels of resistance to thiamethoxam with resistance ratios ranging from 6.66- to 9.52-fold. Expression analyses indicated that CYP9Z140, CYP9AY1, and UGT321AP1 were significantly upregulated in thiamethoxam-resistant populations compared with susceptible populations. The expression of all three genes also increased significantly after thiamethoxam treatment compared with the control. Spatiotemporal expression patterns showed that the highest expression of CYP9Z140 and CYP9AY1 occurred in pupae and the midgut, whereas UGT321AP1 was highly expressed in adults and Malpighian tubules. Knocking down all three genes individually or simultaneously using RNA interference increased the sensitivity of adult L. decemlineata to thiamethoxam. These results suggest that overexpression of CYP9Z140, CYP9AY1, and UGT321AP1 contributes to the development of thiamethoxam resistance in L. decemlineata and provides a scientific basis for improving new resistance management of CPB.
- 相关文献
作者其他论文 更多>>
-
Rapid and Sensitive On-Site Nucleic Acid Detection of Three Main Fusarium Pathogens of Maize Stalk Rot Based on RPA-CRISPR/Cas12a
作者：Jiang, Fan;Jiang, Fan;Ding, Xinhua;Wang, Xiaowu;Fu, Kaiyun;Jia, Zunzun;Guo, Wenchao;Jiang, Fan;Liang, Liang
关键词：CRISPR/Cas12a;
Fusarium graminearum ;Fusarium proliferatum ;Fusarium verticillioides ; recombinase polymerase amplification (RPA) -
Chromosome-level genome assembly of Z strain European corn borer Ostrinia nubilalis (Lepidoptera: Crambidae)
作者：Ding, Xinhua;Wang, Xiaowu;Fu, Kaiyun;Jia, Zunzun;Rouzi, Aerziguli;Ahmat, Tursun;Guo, Wenchao;Zhang, Yue;Wang, Zhihui
关键词：
-
Trichogramma chilonis is a promising biocontrol agent against Tuta absoluta in China: results from laboratory and greenhouse experiments
作者：Li, Xiaowei;Chen, Tingting;Chen, Limin;Ullah, Farman;Yi, Songwang;Pan, Yuhan;Zhou, Shuxing;Lu, Yaobin;Chen, Tingting;Li, Yuan-Xi;Ren, Jie;Guo, Wenchao;Fu, Kaiyun;Lu, Yaobin
关键词：South American tomato pinworm; augmentative biological control; Trichogramma dendrolimi; T. japonicum; T. ostriniae; parasitism rate; release density; release pattern
-
Spinosad-induced intergenerational sublethal effects on Tuta absoluta : biological traits and related genes expressions
作者：Ullah, Farman;Hafeez, Muhammad;Zhou, Shuxing;Zhang, Zhijun;Huang, Jun;Li, Xiaowei;Lu, Yaobin;Guencan, Ali;Gul, Hina;Wang, Yaru;Lu, Yaobin;Ghramh, Hamed A.;Ghramh, Hamed A.;Guo, Wenchao;Fu, Kaiyun;Desneux, Nicolas
关键词：biopesticide; South American tomato pinworm; insecticide toxicity; demographic parameters; age-stage; two- sex life table
-
Datasets of fungal diversity and pseudo-chromosomal genomes of mangrove rhizosphere soil in China
作者：Chen, Jianwei;Peng, Ling;Zhou, Changhao;Li, Liangwei;Ge, Qijin;Shi, Chengcheng;Guo, Wenjie;Guo, Tianci;Fan, Guangyi;Chen, Jianwei;Zhou, Changhao;Fan, Guangyi;Kristiansen, Karsten;Chen, Jianwei;Zhou, Changhao;Fan, Guangyi;Kristiansen, Karsten;Chen, Jianwei;Kristiansen, Karsten;Jiang, Ling;Zhang, Zhidong;Fan, Guangyi;Zhang, Wenwei;Kristiansen, Karsten;Jia, Yangyang
关键词：
-
First report of the complete mitochondrial genome of Carpomya pardalina (Bigot) (Diptera: Tephritidae) and phylogenetic relationships with other Tephritidae
作者：Guo, Xianting;Deng, Jianyu;Rao, Qiong;Wang, Hualing;Fu, Kaiyun;Ding, Xinhua;Guo, Wenchao
关键词：Mitochondrial genome; Phylogenetic analysis; Tephritidae; Carpomya pardalina
-
Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae)
作者：Wang, Xiaowu;Yuan, Zihan;Zhan, Faqiang;Zhou, Liuyan;Dai, Jinping;Wang, Zhifang;Xie, Yuqing;Yang, Xinping;Wang, Xiaowu;Ding, Xinhua;Jia, Zunzun;Fu, Kaiyun;Guo, Wenchao;Li, Haiqiang;Ding, Xinhua;Jia, Zunzun;Fu, Kaiyun;Guo, Wenchao;Li, Haiqiang
关键词：Aspergillus nomius; ostrinia furnacalis; lethal efficacy; infection processes; cuticle; extracellular enzymes activity
