您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Fine mapping and gene silencing pinpoint Capana10g002229 as a strong candidate gene regulating the deciduous character of ripe pepper fruit (Capsicum spp.)

文献类型： 外文期刊

作者： Hu, Fang 1 ; Dong, Jichi 1 ; Zhang, Shaobin 2 ; Song, Zhao 3 ; Guan, Wendong 1 ; Yuan, Fanchong 1 ; Zhong, Jian 1 ; Liu, Jia 1 ; Hu, Kailin 1 ; Cheng, Jiaowen 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Guangdong Vegetables Engn Res Ctr, Key Lab Biol & Genet Improvement Hort Crops South, Guangzhou, Peoples R China

2.Shaoguan Univ, Henry Fok Sch Biol & Agr, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan 512023, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 5 期

页码：

收录情况： SCI

摘要： Key message The pepper S locus, which controls the deciduous character of ripe fruit, was first fine mapped into an interval with a physical length of similar to 38.03 kb on chromosome P10. Capana10g002229, encoding a polygalacturonase, was proposed as a strong candidate gene based on sequence comparison, expression pattern analysis and virus-induced gene silencing (VIGS). The deciduous character of ripe fruit, which is controlled by the dominant S locus, is a domesticated trait with potential value in the pepper processing industry (Capsicum spp.). However, the gene associated with the S locus has not been identified. Here, one major QTL designated S10.1 was detected by using the F-2 population (n = 155) derived from BA3 (Capsicum annuum) x YNXML (Capsicum frutescens) and was further verified in an intraspecific backcross population (n = 254) derived from the cross between BB3 (C. annuum) and its wild relative Chiltepin (C. annuum var. glabriusculum) with BB3 as the recurrent parent. Then, a large BC1F2 population derived from the self-pollination of BB3 x (BB3 x Chiltepin) individuals and comprising 4217 individuals was used to screen the recombinants, and the S locus was ultimately delimited into a 38.03-kb region on chromosome P10 harbouring four annotated genes. Capana10g002229, encoding a polygalacturonase (PG), was proposed as the best candidate gene for S based on sequence comparison and expression pattern analyses. Downregulation of Capana10g002229 in fruits through VIGS significantly delayed fruit softening and abscission from the fruit-receptacle junction. Taken together, the results show that Capana10g002229 could be regarded as a strong candidate gene associated with the S locus in pepper. These findings not only lay a foundation for deciphering the molecular mechanisms underlying pepper domestication but also provide a strategy for genetic improvement of the deciduous character of ripe fruit using a marker-assisted selection approach.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台