您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Acetylation of inorganic pyrophosphatase by S-RNase signaling induces pollen tube tip swelling by repressing pectin methylesterase

文献类型： 外文期刊

作者： Tang, Chao 1 ; Wang, Peng 1 ; Zhu, Xiaoxuan 2 ; Qi, Kaijie 1 ; Xie, Zhihua 1 ; Zhang, Hao 1 ; Li, Xiaoqiang 1 ; Gao, Hongru 1 ; Gu, Tingting 1 ; Gu, Chao 1 ; Li, Shan 1 ; de Graaf, Barend H. J. 3 ; Zhang, Shaoling 1 ; Wu, Juyou 1 ;

作者机构： 1.Nanjing Agr Univ, Sanya Inst, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Jiangsu Engn Res Ctr Pear, Nanjing 210014, Peoples R China

3.Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, Wales

4.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Self-incompatibility (SI) is a widespread genetically determined system in flowering plants that prevents self-fertilization to promote gene flow and limit inbreeding. S-RNase-based SI is characterized by the arrest of pollen tube growth through the pistil. Arrested pollen tubes show disrupted polarized growth and swollen tips, but the underlying molecular mechanism is largely unknown. Here, we demonstrate that the swelling at the tips of incompatible pollen tubes in pear (Pyrus bretschneideri [Pbr]) is mediated by the SI-induced acetylation of the soluble inorganic pyrophosphatase (PPA) PbrPPA5. Acetylation at Lys-42 of PbrPPA5 by the acetyltransferase GCN5-related N-acetyltransferase 1 (GNAT1) drives accumulation of PbrPPA5 in the nucleus, where it binds to the transcription factor PbrbZIP77, forming a transcriptional repression complex that inhibits the expression of the pectin methylesterase (PME) gene PbrPME44. The function of PbrPPA5 as a transcriptional repressor does not require its PPA activity. Downregulating PbrPME44 resulted in increased levels of methyl-esterified pectins in growing pollen tubes, leading to swelling at their tips. These observations suggest a mechanism for PbrPPA5-driven swelling at the tips of pollen tubes during the SI response. The targets of PbrPPA5 include genes encoding cell wall-modifying enzymes, which are essential for building a continuous sustainable mechanical structure for pollen tube growth. In the self-incompatibility response, acetylation of PbrPPA5 enhances the repression of PbrPME44, resulting in the accumulation of methyl-esterified pectins of the pollen tube cell wall and tip swelling.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台