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Identification of Candidate Genes Associated with Trichothecene Biosynthesis in Fusarium graminearum Species Complex Combined with Transcriptomic and Proteomic Analysis

文献类型： 外文期刊

作者： Wang, Jianhua 1 ; Lin, Shanhai 1 ; Zhu, Guanghui 3 ; Zhao, Zhiyong 1 ; Wang, Haoyu 1 ; Zhou, Miaoping 4 ; Zhao, Xingming 5 ; Wu, Aibo 6 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

2.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China

3.Tongji Univ, Dept Comp Sci & Technol, Shanghai 201804, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing 210014, Peoples R China

5.Fudan Univ, Inst Sci & Technol Brain Inspired Intelligence, Shanghai 200433, Peoples R China

6.Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, SIBS UGENT SJTU Joint Lab Mycotoxin Res, CAS Key Lab Nutr Metab & Food Safety, Shanghai 200031, Peoples R China

关键词： Fusarium graminearum species complex; trichothecene; mycotoxin; biosynthesis

期刊名称：MICROORGANISMS （ 影响因子：4.926； 五年影响因子：5.143 ）

ISSN：

年卷期： 2022 年 10 卷 8 期

页码：

收录情况： SCI

摘要： The Fusarium graminearum species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in Fusarium strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix Fusarium GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by FGSG_01403, was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of FGSG_01403 resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the Fusarium graminearum species complex.

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