文献类型： 外文期刊

作者： Feng, Yong ¹ ; Jiang, Mengyun ¹ ; Yu, Weichang ³ ; Zhou, Jiannan ⁵ ;

作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab,M, Shenzhen, Peoples R China

2.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng, Peoples R China

3.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Key Lab Plant Epigenet, Shenzhen, Peoples R China

4.Liaoning Acad Agr Sci, Liaoning Peanut Res Inst, Fuxing, Peoples R China

5.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang, Peoples R China

关键词： small open reading frame; sORFs; ribo-seq; plant; genome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The roles of short/small open reading frames (sORFs) have been increasingly recognized in recent years due to the rapidly growing number of sORFs identified in various organisms due to the development and application of the Ribo-Seq technique, which sequences the ribosome-protected footprints (RPFs) of the translating mRNAs. However, special attention should be paid to RPFs used to identify sORFs in plants due to their small size (similar to 30 nt) and the high complexity and repetitiveness of the plant genome, particularly for polyploidy species. In this work, we compare different approaches to the identification of plant sORFs, discuss the advantages and disadvantages of each method, and provide a guide for choosing different methods in plant sORF studies.