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Identification of Crucial Modules and Genes Associated with Bt Gene Expression in Cotton

文献类型： 外文期刊

作者： Zhao, Guiyuan 1 ; Geng, Zhao 2 ; Liu, Jianguang 2 ; Tian, Haiyan 2 ; Liu, Xu 2 ; An, Zetong 2 ; Zhao, Ning 2 ; Zhang, Hanshuang 2 ; Wu, Liqiang 1 ; Wang, Xingfen 1 ; Wang, Yongqiang 2 ; Zhang, Guiyin 1 ;

作者机构： 1.Hebei Agr Univ, Coll Agron, State Key Lab North China Crop Improvement & Regul, Key Lab Crop Germplasm Resources Hebei, Baoding 071001, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Minist Agr & Rural Affairs, Inst Cotton, Key Lab Cotton Biol & Genet Breeding Huanghuaihai, Shijiazhuang 050051, Peoples R China

关键词： cotton; molecular breeding; Bacillus thuringiensis toxins; WGCNA

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 4 期

页码：

收录情况： SCI

摘要： The expression of Bacillus thuringiensis (Bt) toxins in transgenic cotton confers resistance to insect pests. However, it has been demonstrated that its effectiveness varies among cotton cultivars and different tissues. In this study, we evaluated the expression of Bt protein in 28 cotton cultivars and selected 7 cultivars that differed in Bt protein expression for transcriptome analysis. Based on their Bt protein expression levels, the selected cultivars were categorized into three groups: H (high Bt protein expression), M (moderate expression), and L (low expression). In total, 342, 318, and 965 differentially expressed genes were detected in the H vs. L, M vs. L, and H vs. M comparison groups, respectively. And three modules significantly associated with Bt protein expression were identified by weighted gene co-expression network analysis. Three hub genes were selected to verify their relationships with Bt protein expression using virus-induced gene silencing (VIGS). Silencing GhM_D11G1176, encoding an MYC transcription factor, was confirmed to significantly decrease the expression of Bt protein. The present findings contribute to an improved understanding of the mechanisms that influence Bt protein expression in transgenic cotton.

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