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The Anti-Inflammatory Activities of Sphagnum palustre L. Ethanol Extract to Control Inflammation in RAW264.7 Cells

文献类型： 外文期刊

作者： Wu, Zhi 1 ; Zheng, Jiahui 2 ; Xu, Yang 1 ; Wang, Dairong 1 ; Han, Qingbin 1 ; Liu, Ping 1 ; Liu, Xiaorong 1 ; Zhang, Lanyue 2 ;

作者机构： 1.Guangdong Acad Agr Sci, Environm Hort Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou, Peoples R China

2.Guangdong Univ Technol, Inst Nat Med & Green Chem, Med Food Homol Engn Ctr Guangdong Prov, Sch Biomed & Pharmaceut Sci,Guangdong Prov Key Lab, Guangzhou, Peoples R China

关键词： high-performance liquid chromatography-mass spectrometry; inflammation; macrophage; natural product; Sphagnum palustre L

期刊名称：FLAVOUR AND FRAGRANCE JOURNAL （ 影响因子：2.0； 五年影响因子：2.6 ）

ISSN： 0882-5734

年卷期： 2025 年 40 卷 3 期

页码：

收录情况： SCI

摘要： Discomfort caused by inflammation leads to stress and anxiety in patients and seriously decreases the patients' quality of life. People prefer to use natural products instead of anti-inflammatory drugs because of their low toxicity and side effects. Studies have shown that Sphagnum palustre L. (S. palustre) can be used as medicinal plant, but few studies have focused on its anti-inflammatory effects. This study explored the mechanism of action of the ethanol extract of the peat moss plant S. palustre on lipopolysaccharide-induced inflammation in macrophage RAW264.7 cells. Components in S. palustre ethanol extracts (SPE) were identified by HPLC-MS, which mainly included 4-methoxybenzaldehyde, 4-methoxycinnamaldehyde and oleanolic acid. The effects of different concentrations (6.25-100 mu g/mL) of SPE after 24 h administration were evaluated to establish a cellular inflammation model. Three biological replicates were performed based on each experiment, the MTT assay results showed that a low concentration of SPE promoted cell proliferation marked by Formazan. In a neutral red uptake assay, the SPE group was effectively inhibited the cell phagocytosis rate. With the increase of SPE concentration, intracellular ROS release decreased, which detected by DCFH-DA. Immunofluorescence assay result showed that SPE inhibited the release of reactive oxygen species from macrophages with fluorescent markers and DAPI. SPE inhibited the release of nitric oxide from macrophages as well. What's more, SPE significantly decreased the protein expression of interleukin (IL)-1, IL-6, and nuclear factor (NF)-kappa B according to enzyme-linked immunosorbent and immunocytochemical assays. SPE reduces inflammation in macrophage RAW264.7 cells and thus is a promising natural anti-inflammatory plant.

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