文献类型： 外文期刊

作者： Lu, Xinkun ¹ ; Lu, Yanqing ¹ ; Lin, Yanjin ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Pomol, Fuzhou 350013, Peoples R China

关键词： carotenoid; Citrus grandis L. Osbeck; gene expression; red-pericarp; regulation; RNA-seq

期刊名称：EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE （ 影响因子：1.482； 五年影响因子：1.503 ）

ISSN： 1611-4426

年卷期： 2021 年 86 卷 2 期

页码：

收录情况： SCI

摘要： Carotenoid has an important impact on the nutritional quality and colour development of fruits. Although the knowledge on carotenoid metabolism is well known, the mechanism of regulation of carotenoid accumulation remains unclear. 'Huangbao' pummelo (red-pericarp) is selected from bud mutation of `Guanxi. pummelo (yellow-pericarp). In order to reveal the differences in colour development of fruits between the two cultivars, carotenoid contents and transcriptomic profile were analyzed in the pericarp of ripe fruits from the two pummelo cultivars. Results show that the lycopene content in the pericarp of 'Huangbao' (160.29 mu g g(-1) FW) was significantly higher compared with 'Guanxi' (0.91 mu g g(-1) FW). Lycopene is the main pigment responsible for pericarp colouration. A total of 929 differentially expressed genes (DEGs) were identified. Based on Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, some genes enriched in signaling pathway, carotenoid isomerase activity and carbon fixation in photosynthetic organism pathway may be associated with carotenoid accumulation in the pericarp. Genes encoding for fructose-bisphosphate aldolase, enolase, and pyruvate kinase isozyme were significantly up -regulated in 'Huangbao', while genes annotated as phytoene synthase and prolycopene isomerase were distinctly down-regulated. GibbereBlin-213-dioxygenase (GA20X) gene was especially expressed in 'Huangbao'; however, genes encoding for DELLA protein and auxin transporter were not detected. ERF transcription factors involved in ethylene signaling pathway were down-regulated in 'Huangbao'. These findings are meaningful to reveal that carotenoid precursor metabolism and phytohormone pathways may be involved in the regulation of lycopene accumulation in the pericarp of pummelo fruits.