文献类型： 外文期刊

作者： Lyu, Sunjian ¹ ; Guo, Qi ¹ ; Shen, Weifeng ¹ ; Han, Mingming ¹ ; Xiong, Fulei ¹ ; Dai, Xiaoling ² ; Liu, Li ¹ ; Bu, Weishao ³ ; Lou, Bao ¹ ; Yuan, Julin ⁴ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hydrobiol, State Key Lab Managing Biot & Chem Threats Qual &, 198, Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

2.China Jiliang Univ, Coll Life Sci, 258 Xueyuan St, Hangzhou 310018, Zhejiang, Peoples R China

3.Yunhe Cty Qingjiang Ecol Trionyx Sinensis Breeding, Yunhe Cty 310018, Zhejiang, Peoples R China

4.Zhejiang Inst Freshwater Fisheries, Agr Minist, Key Lab Fish Hlth & Nutr Zhejiang Prov, Key Lab Hlth Freshwater Aquaculture, 999 South Hangchangqiao Rd, Huzhou 313001, Zhejiang, Peoples R China

关键词： Whole transcriptome sequencing; TSHSV; Trionyx sinensis; Non-coding RNAs

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 144 卷

页码：

收录情况： SCI

摘要： Trionyx sinensis Hemorrhagic Syndrome Virus (TSHSV), the first aquatic arterivirus identified in China, causes severe mortality to T. sinensis. In this study, we sought to determine the functions of T. sinensis mRNAs and non-coding RNAs (ncRNAs) that were differentially expressed (DE) over different periods of TSHSV infection of T. sinensis lung. We used RT-qPCR to validate the sequencing results of select RNAs, confirming their reliable and referable nature. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to predict multiple biological functions and signaling pathways in various comparison groups (1-day versus mock, 3-day versus 1-day, and 5-day versus 3-day). Multiple types of differentially expressed RNA, including mRNA, lncRNA, circRNA, and miRNA, were associated with cardiac dysfunction, coagulation abnormalities, and arachidonic acid metabolism at day 1. Pre-inflammatory cytokines and inflammatory factors such as PLA2G4A, cPLA2, gamma-GGT1, TNFRSF14, TCP11L2, PTER CYP2J2 and LTC4S, were noticeably regulated at the same time. On day 3, multiple GO terms and KEGG pathways were implicated, including those related to virus defense, apoptosis, pyroptosis, and inflammatory response. Notably, key genes such as RSAD2, TRIM39, STAT4, CASP1, CASP14, MYD88, CXCL3, CARD11, ZBP1, and ROBO4 exhibited sig-nificant regulation. The lncRNAs and circRNAs that targeted the genes involved in viral recognition (TLR5), apoptosis (CARD11), pyroptosis (ZBP1), inflammatory processes (NEK7, RASGRP4, and SELE) and angiogenesis (ROBO4) exhibited significant regulation. Significantly regulated miRNAs were primarily linked to genes involved in apoptosis (Let-7f-3p, miR-1260a, miR-455-3p), and inflammation (miR-146a, miR-125a, miR-17a, miR-301b, and miR-30a-3p). The findings could advance our understanding of the host immunological response to TSHSV and offer new ideas for developing effective strategies to prevent infection of T. sinensis.