文献类型： 外文期刊

作者： Xiong, Zhuqing ¹ ; Xu, Kai ¹ ; Lin, Zili ⁴ ; Kong, Feng ¹ ; Wang, Qiujun ¹ ; Quan, Yujun ⁵ ; Sha, Qian-qian ⁷ ; Li, Fajin ² ; Zou, Zhuoning ¹ ; Liu, Ling ¹ ; Ji, Shuyan ¹ ; Chen, Yuling ¹⁰ ; Zhang, Hongmei ¹ ; Fang, Jianhuo ³ ; Yu, Guang ¹ ; Liu, Bofeng ¹ ; Wang, Lijuan ¹ ; Wang, Huili ¹¹ ; Deng, Haiteng ¹⁰ ; Yang, Xuerui ⁸ ; Fan, Heng-yu ¹² ; Li, Lei ⁵ ; Xie, Wei ¹ ;

作者机构： 1.Tsinghua Univ, Ctr Stem Cell Biol & Regenerat Med, Sch Life Sci, MOE Key Lab Bioinformat, Beijing, Peoples R China

2.Tsinghua Univ, Peking Univ Tsinghua Univ Natl Inst Biol Sci Join, Sch Life Sci, Beijing, Peoples R China

3.Tsinghua Peking Ctr Life Sci, Beijing, Peoples R China

4.Beijing Univ Agr, Coll Anim Sci & Technol Coll, Beijing, Peoples R China

5.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing, Peoples R China

6.Univ Chinese Acad Sci, Beijing, Peoples R China

7.Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Fertil Preservat Lab, Guangzhou, Peoples R China

8.Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing, Peoples R China

9.Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing, Peoples R China

10.Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China

11.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China

12.Zhejiang Univ, MOE Key Lab Biosyst Homeostasis & Protect, Life Sci Inst, Hangzhou, Peoples R China

13.Zhejiang Univ, Innovat Ctr Cell Signaling Network, Life Sci Inst, Hangzhou, Peoples R China

14.Zhejiang Univ, Sch Med, Sir Run Run Shaw Hosp, Assisted Reprod Unit,Dept Obstet & Gynecol,Key La, Hangzhou, Peoples R China

期刊名称：NATURE CELL BIOLOGY （ 影响因子：28.213； 五年影响因子：28.123 ）

ISSN： 1465-7392

年卷期： 2022 年 24 卷 6 期

页码：

收录情况： SCI

摘要： In mammals, translational control plays critical roles during oocyte-to-embryo transition (OET) when transcription ceases. However, the underlying regulatory mechanisms remain challenging to study. Here, using low-input Ribo-seq (Ribo-lite), we investigated translational landscapes during OET using 30-150 mouse oocytes or embryos per stage. Ribo-lite can also accommodate single oocytes. Combining PAIso-seq to interrogate poly(A) tail lengths, we found a global switch of translatome that closely parallels changes of poly(A) tails upon meiotic resumption. Translation activation correlates with polyadenylation and is supported by polyadenylation signal proximal cytoplasmic polyadenylation elements (papCPEs) in 3 ' untranslated regions. By contrast, translation repression parallels global de-adenylation. The latter includes transcripts containing no CPEs or non-papCPEs, which encode many transcription regulators that are preferentially re-activated before zygotic genome activation. CCR4-NOT, the major de-adenylation complex, and its key adaptor protein BTG4 regulate translation downregulation often independent of RNA decay. BTG4 is not essential for global de-adenylation but is required for selective gene de-adenylation and production of very short-tailed transcripts. In sum, our data reveal intimate interplays among translation, RNA stability and poly(A) tail length regulation underlying mammalian OET. Using an optimized Ribo-seq protocol that is applicable for low-input samples, Xie, Li and colleagues revealed the translation landscape during oocyte-to-embryo transition and in pre-implantation embryos.