Creating a Superior Wx Allele with Temperature-Responsive Amylose Regulation and a Novel Transcriptional Pattern in Rice via CRISPR/Cas9-Mediated Promoter Editing
文献类型： 外文期刊
作者： Yan, Jiali 1 ; Yu, Jiawen 1 ; Shen, Huimin 1 ; Zhou, Lihui 3 ; Chen, Zhuanzhuan 1 ; Fan, Xiaolei 1 ; Li, Qianfeng 1 ; Zhang, Changquan 1 ; Liu, Qing 1 ; Huang, Lichun 1 ; Liu, Qiaoquan 1 ;
作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genom & Mol Breeding, Zhongshan Biol Breeding Lab, Minist Educ,Key Lab Plant Funct Genom,Agr Coll, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops Jian, Jiangsu Key Lab Crop Genet & Physiol, Yangzhou 225009, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Food Crops, Jiangsu High Qual Rice Res & Dev Ctr, Jiangsu Key Lab Agrobiol, Nanjing 210014, Peoples R China
4.Yangzhou Modern Seed Innovat Inst, Gaoyou 225600, Peoples R China
关键词：
期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 14 卷 8 期
页码：
收录情况： SCI
摘要： High quality stands as a pivotal competitive edge in the rice industry. Optimizing amylose content (AC) and the physicochemical properties of endosperm starch by regulating the Wx gene is crucial for enhancing rice grain quality. In this study, we created a novel Wxb-d25 allele by deleting a 25 bp segment (-26 to -2) within the Wx core promoter using CRISPR/Cas9. Compared with the wild type and the previously reported Wxb-i1, Wxb-d25 exhibited no significant changes in agronomic traits. However, its grains displayed temperature-dependent variations in AC and altered transparency and viscosity characteristics, holding the potential to synergistically improve both the eating and cooking quality (ECQ) and appearance quality (AQ) of rice. Further studies demonstrated that this promoter modification, by partially disrupting the transcription initiator, significantly downregulated the original Wx-01 transcript and generated a novel Wx transcript (ONT.7395.1) in Wxb-d25 grains. Despite its low expression abundance, the ONT.7395.1 transcript could be completely processed into mature Wx mRNA. The combined effects of the dual transcripts resulted in significantly increased Wx gene expression and AC in Wxb-d25 grains under conventional cultivation conditions. These findings provide a genetic resource and a theoretical foundation for utilizing the Wxb-d25 allele to improve rice grain quality.
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