Genome-Wide Identification of R2R3-MYB Transcription Factors: Discovery of a "Dual-Function" Regulator of Gypenoside and Flavonol Biosynthesis in Gynostemma pentaphyllum
文献类型： 外文期刊
作者： Huang, Ding 1 ; Ming, Ruhong 1 ; Xu, Shiqiang 3 ; Yao, Shaochang 1 ; Li, Liangbo 1 ; Huang, Rongshao 1 ; Tan, Yong 1 ;
作者机构： 1.Guangxi Univ Chinese Med, Coll Pharm, Nanning, Peoples R China
2.Guangxi Univ Chinese Med, Guangxi Key Lab Zhuang & Yao Ethn Med, Nanning, Peoples R China
3.Guangdong Prov Engn & Technol Res Ctr Conservat &, Guangzhou, Peoples R China
4.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crops Genet & Improvement, Guangzhou, Peoples R China
关键词： R2R3-MYB gene family; Gynostemma pentaphyllum; gypenoside; flavonol; transcription factor
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 12 卷
页码：
收录情况： SCI
摘要： The R2R3-MYB gene family participates in several plant physiological processes, especially the regulation of the biosynthesis of secondary metabolites. However, little is known about the functions of R2R3-MYB genes in Gynostemma pentaphyllum (G. pentaphyllum), a traditional Chinese medicinal herb that is an excellent source of gypenosides (a class of triterpenoid saponins) and flavonoids. In this study, a systematic genome-wide analysis of the R2R3-MYB gene family was performed using the recently sequenced G. pentaphyllum genome. In total, 87 R2R3-GpMYB genes were identified and subsequently divided into 32 subgroups based on phylogenetic analysis. The analysis was based on conserved exon-intron structures and motif compositions within the same subgroup. Collinearity analysis demonstrated that segmental duplication events were majorly responsible for the expansion of the R2R3-GpMYB gene family, and Ka/Ks analysis indicated that the majority of the duplicated R2R3-GpMYB genes underwent purifying selection. A combination of transcriptome analysis and quantitative reverse transcriptase-PCR (qRT-PCR) confirmed that Gynostemma pentaphyllum myeloblastosis 81 (GpMYB81) along with genes encoding gypenoside and flavonol biosynthetic enzymes exhibited similar expression patterns in different tissues and responses to methyl jasmonate (MeJA). Moreover, GpMYB81 could bind to the promoters of Gynostemma pentaphyllum farnesyl pyrophosphate synthase 1 (GpFPS1) and Gynostemma pentaphyllum chalcone synthase (GpCHS), the key structural genes of gypenoside and flavonol biosynthesis, respectively, and activate their expression. Altogether, this study highlights a novel transcriptional regulatory mechanism that suggests that GpMYB81 acts as a "dual-function " regulator of gypenoside and flavonol biosynthesis in G. pentaphyllum.
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